A Biometric Model for Mineralization of Type-I Collagen Fibrils

Shasha Yao, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

Bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In-vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is selfassembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimension (2D) and three-dimension (3D) stochastic optical reconstruction microscopy (STORM), which enables in-situ and high resolution visualization of the process.
Original languageEnglish
Title of host publicationCollagen
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages39-54
ISBN (Electronic)978-1-4939-9095-5
ISBN (Print)978-1-4939-9094-8
DOIs
Publication statusE-pub ahead of print - 7 Mar 2019

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