A Biometric Model for Mineralization of Type-I Collagen Fibrils

Shasha Yao; Changyu Shao; Fabio Nudelman; Nico A. J. M. Sommerdijk; Ruikang Tang

doi:10.1007/978-1-4939-9095-5_4

A Biometric Model for Mineralization of Type-I Collagen Fibrils

Shasha Yao, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In-vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is selfassembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimension (2D) and three-dimension (3D) stochastic optical reconstruction microscopy (STORM), which enables in-situ and high resolution visualization of the process.

Original language	English
Title of host publication	Collagen
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	39-54
ISBN (Electronic)	978-1-4939-9095-5
ISBN (Print)	978-1-4939-9094-8
DOIs	https://doi.org/10.1007/978-1-4939-9095-5_4
Publication status	E-pub ahead of print - 7 Mar 2019

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10.1007/978-1-4939-9095-5_4

book chapter - Nudelman - A biomimetic model for collagenAccepted author manuscript, 728 KB
book chapter - Nudelman - Figures--A biomimetic model for collagenAccepted author manuscript, 1.49 MBLicence: Creative Commons: Attribution (CC-BY)

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title = "A Biometric Model for Mineralization of Type-I Collagen Fibrils",

abstract = "Bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In-vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is selfassembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimension (2D) and three-dimension (3D) stochastic optical reconstruction microscopy (STORM), which enables in-situ and high resolution visualization of the process. ",

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AB - Bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In-vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is selfassembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimension (2D) and three-dimension (3D) stochastic optical reconstruction microscopy (STORM), which enables in-situ and high resolution visualization of the process.

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A Biometric Model for Mineralization of Type-I Collagen Fibrils

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