A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death

Nicole D. Barth, Lorena Mendive‐tapia, Ramon Subiros‐funosas, Ouldouz Ghashghaei, Rodolfo Lavilla, Laura Maiorino, Xue‐yan He, Ian Dransfield, Mikala Egeblad, Marc Vendrell

Research output: Contribution to journalArticlepeer-review

Abstract

The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high-resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. Here we report the design and optimisation of the fluorogenic probe Apotracker Red for real-time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.
Original languageEnglish
JournalAngewandte Chemie International Edition
Volume61
Issue number5
DOIs
Publication statusPublished - 26 Jan 2022

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