A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis

F. J. Klein, P Mahr, M Galova, S B Buonomo, C Michaelis, K Nairz, Kim Nasmyth

Research output: Contribution to journalArticlepeer-review

Abstract

A multisubunit complex, called cohesin, containing Smc1p, Smc3p, Scc1p, and Scc3p, is required for sister chromatid cohesion in mitotic cells. We show here that Smc3p and a meiotic version of Scc1p called Rec8p are required for cohesion between sister chromatids, for formation of axial elements, for reciprocal recombination, and for preventing hyperresection of double-strand breaks during meiosis. Both Rec8p and Smc3p colocalize with chromosome cores independently of synapsis during prophase I and largely disappear from chromosome arms after pachytene but persist in the neighborhood of centromeres until the onset of anaphase II. The eukaryotic cell's cohesion apparatus is required both for the repair of recombinogenic lesions and for chromosome segregation and therefore appears to lie at the heart of the meiotic process.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalCell
Volume98
Issue number1
DOIs
Publication statusPublished - 9 Jul 1999

Keywords

  • Anaphase
  • Animals
  • Cell Cycle
  • Cell Cycle Proteins
  • Centromere
  • Chondroitin Sulfate Proteoglycans
  • Chromatids
  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • Humans
  • Meiosis
  • Nuclear Proteins
  • Phosphoproteins
  • Phylogeny
  • Recombination, Genetic
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

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