A central role for P48/45 in malaria parasite male gamete fertility

M R van Dijk; C J Janse; Joanne Thompson; A P Waters; J A Braks; H J Dodemont; H G Stunnenberg; G J van Gemert; R W Sauerwein; W Eling

A central role for P48/45 in malaria parasite male gamete fertility

M R van Dijk, C J Janse, Joanne Thompson, A P Waters, J A Braks, H J Dodemont, H G Stunnenberg, G J van Gemert, R W Sauerwein, W Eling

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.

Original language	English
Pages (from-to)	153-64
Number of pages	12
Journal	Cell
Volume	104
Issue number	1
Publication status	Published - 2001

Cite this

@article{77fe2b2b04d24e2f9828901d53dd1248,

title = "A central role for P48/45 in malaria parasite male gamete fertility",

abstract = "Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.",

author = "{van Dijk}, {M R} and Janse, {C J} and Joanne Thompson and Waters, {A P} and Braks, {J A} and Dodemont, {H J} and Stunnenberg, {H G} and {van Gemert}, {G J} and Sauerwein, {R W} and W Eling",

year = "2001",

language = "English",

volume = "104",

pages = "153--64",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - A central role for P48/45 in malaria parasite male gamete fertility

AU - van Dijk, M R

AU - Janse, C J

AU - Thompson, Joanne

AU - Waters, A P

AU - Braks, J A

AU - Dodemont, H J

AU - Stunnenberg, H G

AU - van Gemert, G J

AU - Sauerwein, R W

AU - Eling, W

PY - 2001

Y1 - 2001

N2 - Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.

AB - Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.

M3 - Article

C2 - 11163248

VL - 104

SP - 153

EP - 164

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -

A central role for P48/45 in malaria parasite male gamete fertility

Abstract

Fingerprint

Cite this