A complex interplay of positive and negative elements is responsible for the different transcriptional activity of liver NF1 variants

Paolo Monaci, Maurizio Nuzzo, Susanne Stämpfli, David Tollervey, Vincenzo De Simone, Alfredo Nicosia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A full-length cDNA of the rat liver Nuclear Factor 1 (NF1L21) has been cloned and expressed in S. cerevisiae to analyse the architecture of its activation domain. NF1L21 displays a specific DNA-binding activity, as well as the ability to activate transcription from an artificial NF1-responsive promoter in yeast. Interaction of two or more NF1L21 molecules with multiple sites on the same promoter activated transcription in a synergistic fashion. Functional analysis of the activation domain of NF1L21 reveals a tripartite structure. Two distinct positive elements are required for NF1L21-mediated transcription activation. A proline-rich element sandwiched between these two positive domains attenuates their transactivation potential. A shorter NF1L variant (NF1L4) in which the distal positive element is replaced by a different sequence was also isolated. NF1L4 displays the same DNA-binding activity and dimerisation properties as NF1L21, but is unable to activate transcription in yeast.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalMolecular biology reports
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Oct 1995

Keywords

  • nuclear factor I
  • proline-rich domain
  • transcription activator
  • yeast

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