A conserved myotubularin-related phosphatase regulates autophagy by maintaining autophagic flux

Elizabeth A. Allen, Clelia Amato, Tina M. Fortier, Panagiotis Velentzas, Will Wood, Eric H. Baehrecke

Research output: Contribution to journalArticlepeer-review

Abstract

Macroautophagy (autophagy) targets cytoplasmic cargoes to the lysosome for degradation. Like all vesicle trafficking, autophagy relies on phosphoinositide identity, concentration and localization to execute multiple steps in this catabolic process. Here we screen for phosphoinositide phosphatases that influence autophagy in Drosophila, and identify CG3530. CG3530 is homologous to the human MTMR6 subfamily of myotubularin-related 3-phosphatases, and therefore we named it dMtmr6. dMtmr6, which is required for development and viability in Drosophila, functions as a regulator of autophagic flux in multiple Drosophila cell types. The MTMR6 family member MTMR8 has a similar function in autophagy of higher animal cells. Decreased dMtmr6 and MTMR8 function results in autophagic vesicle accumulation and influences endolysosomal homeostasis.

Original languageEnglish
Article numbere201909073
JournalJournal of Cell Biology
Volume219
Issue number11
Early online date11 Sep 2020
DOIs
Publication statusPublished - 2 Nov 2020

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