A constitutive PR-1 :: luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens

S L  Murray; N  Adams; D J  Kliebenstein; G J  Loake; K J  Denby

A constitutive PR-1 :: luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens

S L Murray, N Adams, D J Kliebenstein, G J Loake, K J Denby

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in Arabi-dolpsis. To understand this defence signalling network further, we identified mutants that expressed the marker gene PR-1-luciferase in the absence of pathogen infection. These cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, Pseudomonas syringae pv. tomato and Peronospora parasitica NOCO2, and the necrotrophic pathogen Botrytis cinerea. We further characterized cir3, which displays enhanced resistance only to the necrotrophic pathogen. Cir3-mediated resistance to B. cinerea is dependent on accumulated salicylic acid and a functional EIN2 protein.

Original language	English
Pages (from-to)	31-41
Number of pages	11
Journal	Molecular Plant Pathology
Volume	6
Issue number	1
Publication status	Published - Jan 2005

Cite this

@article{759751d2815a4c1bab23704e59aee14f,

title = "A constitutive PR-1 :: luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens",

abstract = "A complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in Arabi-dolpsis. To understand this defence signalling network further, we identified mutants that expressed the marker gene PR-1-luciferase in the absence of pathogen infection. These cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, Pseudomonas syringae pv. tomato and Peronospora parasitica NOCO2, and the necrotrophic pathogen Botrytis cinerea. We further characterized cir3, which displays enhanced resistance only to the necrotrophic pathogen. Cir3-mediated resistance to B. cinerea is dependent on accumulated salicylic acid and a functional EIN2 protein.",

author = "Murray, {S L} and N Adams and Kliebenstein, {D J} and Loake, {G J} and Denby, {K J}",

year = "2005",

month = jan,

language = "English",

volume = "6",

pages = "31--41",

journal = "Molecular Plant Pathology",

issn = "1464-6722",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - A constitutive PR-1 :: luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens

AU - Murray, S L

AU - Adams, N

AU - Kliebenstein, D J

AU - Loake, G J

AU - Denby, K J

PY - 2005/1

Y1 - 2005/1

N2 - A complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in Arabi-dolpsis. To understand this defence signalling network further, we identified mutants that expressed the marker gene PR-1-luciferase in the absence of pathogen infection. These cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, Pseudomonas syringae pv. tomato and Peronospora parasitica NOCO2, and the necrotrophic pathogen Botrytis cinerea. We further characterized cir3, which displays enhanced resistance only to the necrotrophic pathogen. Cir3-mediated resistance to B. cinerea is dependent on accumulated salicylic acid and a functional EIN2 protein.

AB - A complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in Arabi-dolpsis. To understand this defence signalling network further, we identified mutants that expressed the marker gene PR-1-luciferase in the absence of pathogen infection. These cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, Pseudomonas syringae pv. tomato and Peronospora parasitica NOCO2, and the necrotrophic pathogen Botrytis cinerea. We further characterized cir3, which displays enhanced resistance only to the necrotrophic pathogen. Cir3-mediated resistance to B. cinerea is dependent on accumulated salicylic acid and a functional EIN2 protein.

M3 - Article

SN - 1464-6722

VL - 6

SP - 31

EP - 41

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

IS - 1

ER -

A constitutive PR-1 :: luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens

Abstract

Fingerprint

Cite this