A cooperative polymer-DNA microarray approach to biomaterial investigation

Salvatore Pernagallo; Juan Jose Diaz-Mochon; Mark Bradley

doi:10.1039/b808363k

A cooperative polymer-DNA microarray approach to biomaterial investigation

Salvatore Pernagallo, Juan Jose Diaz-Mochon, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.

Original language	English
Pages (from-to)	397-403
Number of pages	7
Journal	Lab on a Chip
Volume	9
Issue number	3
DOIs	https://doi.org/10.1039/b808363k
Publication status	Published - 2009

Keywords

GENE-EXPRESSION
CELL INTERACTION
STEM-CELLS
TISSUE
SCALE
LEUKEMIA
SURFACE
PROLIFERATION
SCIENCE
BINDING

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10.1039/b808363k

Cite this

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title = "A cooperative polymer-DNA microarray approach to biomaterial investigation",

abstract = "In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.",

keywords = "GENE-EXPRESSION, CELL INTERACTION, STEM-CELLS, TISSUE, SCALE, LEUKEMIA, SURFACE, PROLIFERATION, SCIENCE, BINDING",

author = "Salvatore Pernagallo and Diaz-Mochon, {Juan Jose} and Mark Bradley",

year = "2009",

doi = "10.1039/b808363k",

language = "English",

volume = "9",

pages = "397--403",

journal = "Lab on a Chip",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

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T1 - A cooperative polymer-DNA microarray approach to biomaterial investigation

AU - Pernagallo, Salvatore

AU - Diaz-Mochon, Juan Jose

AU - Bradley, Mark

PY - 2009

Y1 - 2009

N2 - In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.

AB - In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.

KW - GENE-EXPRESSION

KW - CELL INTERACTION

KW - STEM-CELLS

KW - TISSUE

KW - SCALE

KW - LEUKEMIA

KW - SURFACE

KW - PROLIFERATION

KW - SCIENCE

KW - BINDING

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U2 - 10.1039/b808363k

DO - 10.1039/b808363k

M3 - Article

VL - 9

SP - 397

EP - 403

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 3

ER -

A cooperative polymer-DNA microarray approach to biomaterial investigation

Abstract

Keywords

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Biocompatible polymer micro-arrays for cellular growth, stem cell manipulation, cellular release and identification and high-content screening

Platform: High- Throughput chemical biology for efficient cellular delivery

Cite this

A cooperative polymer-DNA microarray approach to biomaterial investigation

Abstract

Keywords

Access to Document

Other files and links

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Projects

Biocompatible polymer micro-arrays for cellular growth, stem cell manipulation, cellular release and identification and high-content screening

Platform: High- Throughput chemical biology for efficient cellular delivery

Cite this