A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep

Clare Pridans, Gemma Davis, Kristin Sauter, Zofia Lisowski, Yolanda Corripio-Miyar, Anna Raper, Lucas Lefevre, Rachel Young, Mary E. McCulloch, Simon Lillico, Elspeth Milne, Christopher Whitelaw, David Hume

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of Colony stimulating factor 1 receptor (Csf1r) in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element (FIRE) for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse FIRE and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate and non-classical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the non-classical subset. As Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.
Original languageEnglish
Pages (from-to)2297-2305
JournalJournal of Immunology
Volume197
Issue number6
Early online date12 Aug 2016
DOIs
Publication statusPublished - 15 Sep 2016

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