A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep

Clare Pridans; Gemma Davis; Kristin Sauter; Zofia Lisowski; Yolanda Corripio-Miyar; Anna Raper; Lucas Lefevre; Rachel Young; Mary E. McCulloch; Simon Lillico; Elspeth Milne; Christopher Whitelaw; David Hume

doi:10.4049/jimmunol.1502336

A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep

Clare Pridans, Gemma Davis, Kristin Sauter, Zofia Lisowski, Yolanda Corripio-Miyar, Anna Raper, Lucas Lefevre, Rachel Young, Mary E. McCulloch, Simon Lillico, Elspeth Milne, Christopher Whitelaw, David Hume

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Expression of Colony stimulating factor 1 receptor (Csf1r) in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element (FIRE) for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse FIRE and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate and non-classical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the non-classical subset. As Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.

Original language	English
Pages (from-to)	2297-2305
Journal	The Journal of Immunology
Volume	197
Issue number	6
Early online date	12 Aug 2016
DOIs	https://doi.org/10.4049/jimmunol.1502336
Publication status	Published - 15 Sept 2016

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10.4049/jimmunol.1502336

A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep
This is an open-access article distributed under the terms of the CC-BY 3.0 Unported license.
Final published version, 1.79 MBLicence: Creative Commons: Attribution (CC-BY)

Functional Annotation of the Sheep Genome
Hume, D., Archibald, A., Morrison, I., Summers, K. & Watson, M.
BBSRC
16/10/13 → 3/05/17
Project: Research
Control of development and reproductive traits
Burdon, T., Argyle, D., Ashworth, C., Beard, P., Brunton, P., Burt, D., Clinton, M., Dunn, I., Farquharson, C., Headon, D., Hocking, P., Hohenstein, P., Hume, D., Jackson, I., McColl, B., McGrew, M., McLachlan, G., Sang, H., Summers, K. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research

Simon Lillico
- simon.lillicoroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Core Scientist (Research Fellow)
- Edinburgh Imaging
Person: Academic: Research Active
Zofia Lisowski
- Royal (Dick) School of Veterinary Studies - Senior Lecturer
Person: Academic: Research Active
Clare Pridans
- Deanery of Clinical Sciences - Research Fellow
- Centre for Inflammation Research
Person: Academic: Research Active

Cite this

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title = "A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep",

abstract = "Expression of Colony stimulating factor 1 receptor (Csf1r) in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element (FIRE) for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse FIRE and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate and non-classical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the non-classical subset. As Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model. ",

author = "Clare Pridans and Gemma Davis and Kristin Sauter and Zofia Lisowski and Yolanda Corripio-Miyar and Anna Raper and Lucas Lefevre and Rachel Young and McCulloch, {Mary E.} and Simon Lillico and Elspeth Milne and Christopher Whitelaw and David Hume",

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day = "15",

doi = "10.4049/jimmunol.1502336",

language = "English",

volume = "197",

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journal = "The Journal of Immunology",

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T1 - A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep

AU - Pridans, Clare

AU - Davis, Gemma

AU - Sauter, Kristin

AU - Lisowski, Zofia

AU - Corripio-Miyar, Yolanda

AU - Raper, Anna

AU - Lefevre, Lucas

AU - Young, Rachel

AU - McCulloch, Mary E.

AU - Lillico, Simon

AU - Milne, Elspeth

AU - Whitelaw, Christopher

AU - Hume, David

PY - 2016/9/15

Y1 - 2016/9/15

N2 - Expression of Colony stimulating factor 1 receptor (Csf1r) in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element (FIRE) for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse FIRE and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate and non-classical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the non-classical subset. As Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.

AB - Expression of Colony stimulating factor 1 receptor (Csf1r) in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element (FIRE) for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse FIRE and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate and non-classical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the non-classical subset. As Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.

U2 - 10.4049/jimmunol.1502336

DO - 10.4049/jimmunol.1502336

M3 - Article

SN - 0022-1767

VL - 197

SP - 2297

EP - 2305

JO - The Journal of Immunology

JF - The Journal of Immunology

IS - 6

ER -

A Csf1r-EGFP transgene provides a novel marker for monocyte subsets in sheep

Abstract / Description of output

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Projects

Functional Annotation of the Sheep Genome

Control of development and reproductive traits

Profiles

Simon Lillico

Zofia Lisowski

Clare Pridans

Cite this