A disposable acetylcholinesterase-based electrode biosensor to detect anatoxin-a(s) in water

F Villatte, H Schulze, R D Schmid, Till Bachmann

Research output: Contribution to journalArticlepeer-review

Abstract

Anatoxin-a(s) is a hazardous toxin released by cyanobacteria during bacterial blooms. A simple and fast method to detect this hazardous compound using a biosensor based on the electrochemical detection of the activity of acetylcholinesterase was developed. Among several acetylcholinesterases, electric eel enzyme was found to be the most sensitive to anatoxin-a(s) and was thus used to build disposable amperometric sensors. The system displayed a detection limit of 1 microg/L anatoxin-a(s). No unspecific effect was noticed with real water samples but spiked toxin was accurately detected. Oxime reactivation was used to discriminate between the toxin and potential insecticides present in the sample.
Original languageEnglish
Pages (from-to)322-6
Number of pages5
JournalAnalytical and bioanalytical chemistry
Volume372
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • Acetylcholinesterase
  • Animals
  • Bacterial Toxins
  • Biosensing Techniques
  • Cholinesterase Inhibitors
  • Disposable Equipment
  • Electrodes
  • Electrophorus
  • Enzymes, Immobilized
  • Marine Toxins
  • Microcystins
  • Tropanes

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