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Abstract
DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.
Original language | English |
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Pages (from-to) | 1873-1879 |
Number of pages | 7 |
Journal | Analyst |
Volume | 134 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2009 |
Keywords / Materials (for Non-textual outputs)
- 2-Aminopurine
- Base Pair Mismatch
- DNA
- DNA Probes
- Fluorescence Resonance Energy Transfer
- Nucleotides
- Spectrometry, Fluorescence
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Dive into the research topics of 'A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets'. Together they form a unique fingerprint.Projects
- 1 Finished
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Beacon Project
Ghazal, P. (Principal Investigator), Crain, J. (Co-investigator), Mount, A. (Co-investigator), Walton, A. (Co-investigator) & Terry, J. (Researcher)
UK central government bodies/local authorities, health and hospital authorities
1/04/03 → 31/03/07
Project: Research