A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

Colin J Campbell, Christopher P Mountford, Helene C Stoquert, Amy H Buck, Paul Dickinson, Elena Ferapontova, Jonathan G Terry, John S Beattie, Anthony J Walton, Jason Crain, Peter Ghazal, Andrew R Mount

Research output: Contribution to journalArticlepeer-review

Abstract

DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.
Original languageEnglish
Pages (from-to)1873-1879
Number of pages7
JournalAnalyst
Volume134
Issue number9
DOIs
Publication statusPublished - 2009

Keywords

  • 2-Aminopurine
  • Base Pair Mismatch
  • DNA
  • DNA Probes
  • Fluorescence Resonance Energy Transfer
  • Nucleotides
  • Spectrometry, Fluorescence

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