A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

Colin J Campbell; Christopher P Mountford; Helene C Stoquert; Amy H Buck; Paul Dickinson; Elena Ferapontova; Jonathan G Terry; John S Beattie; Anthony J Walton; Jason Crain; Peter Ghazal; Andrew R Mount

doi:10.1039/b900325h

A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

Colin J Campbell, Christopher P Mountford, Helene C Stoquert, Amy H Buck, Paul Dickinson, Elena Ferapontova, Jonathan G Terry, John S Beattie, Anthony J Walton, Jason Crain, Peter Ghazal, Andrew R Mount

Research output: Contribution to journal › Article › peer-review

Abstract

DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.

Original language	English
Pages (from-to)	1873-1879
Number of pages	7
Journal	Analyst
Volume	134
Issue number	9
DOIs	https://doi.org/10.1039/b900325h
Publication status	Published - 2009

Keywords

2-Aminopurine
Base Pair Mismatch
DNA
DNA Probes
Fluorescence Resonance Energy Transfer
Nucleotides
Spectrometry, Fluorescence

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10.1039/b900325h

DNA nanoswitch mismatch discrimination investigated using steady-state and time-resolved fluorescence of 2-amino purine.Accepted author manuscript, 511 KB

http://pubs.rsc.org/en/Content/ArticleLanding/2009/AN/b900325h

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1 Finished

Beacon Project
Ghazal, P., Crain, J., Mount, A., Walton, A. & Terry, J.
UK central government bodies/local authorities, health and hospital authorities
1/04/03 → 31/03/07
Project: Research

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Campbell, C. J., Mountford, C. P., Stoquert, H. C., Buck, A. H., Dickinson, P., Ferapontova, E., Terry, J. G., Beattie, J. S., Walton, A. J., Crain, J., Ghazal, P., & Mount, A. R. (2009). A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets. Analyst, 134(9), 1873-1879. https://doi.org/10.1039/b900325h

Campbell, Colin J ; Mountford, Christopher P ; Stoquert, Helene C ; Buck, Amy H ; Dickinson, Paul ; Ferapontova, Elena ; Terry, Jonathan G ; Beattie, John S ; Walton, Anthony J ; Crain, Jason ; Ghazal, Peter ; Mount, Andrew R. / A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets. In: Analyst. 2009 ; Vol. 134, No. 9. pp. 1873-1879.

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title = "A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets",

abstract = "DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.",

keywords = "2-Aminopurine, Base Pair Mismatch, DNA, DNA Probes, Fluorescence Resonance Energy Transfer, Nucleotides, Spectrometry, Fluorescence",

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doi = "10.1039/b900325h",

language = "English",

volume = "134",

pages = "1873--1879",

journal = "Analyst",

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publisher = "Royal Society of Chemistry",

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A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets. / Campbell, Colin J; Mountford, Christopher P; Stoquert, Helene C; Buck, Amy H ; Dickinson, Paul; Ferapontova, Elena; Terry, Jonathan G; Beattie, John S; Walton, Anthony J; Crain, Jason; Ghazal, Peter ; Mount, Andrew R.

In: Analyst, Vol. 134, No. 9, 2009, p. 1873-1879.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

AU - Campbell, Colin J

AU - Mountford, Christopher P

AU - Stoquert, Helene C

AU - Buck, Amy H

AU - Dickinson, Paul

AU - Ferapontova, Elena

AU - Terry, Jonathan G

AU - Beattie, John S

AU - Walton, Anthony J

AU - Crain, Jason

AU - Ghazal, Peter

AU - Mount, Andrew R

PY - 2009

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AB - DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.

KW - 2-Aminopurine

KW - Base Pair Mismatch

KW - DNA

KW - DNA Probes

KW - Fluorescence Resonance Energy Transfer

KW - Nucleotides

KW - Spectrometry, Fluorescence

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DO - 10.1039/b900325h

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SP - 1873

EP - 1879

JO - Analyst

JF - Analyst

SN - 1364-5528

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ER -

A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targets

Abstract

Keywords

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