A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase

D Highton; D Chitnis; S Brigadoi; P Phan; I Tachtsidis; R Cooper; N Everdell; J Hebden; M Smith; C E Elwell

doi:10.1007/978-3-319-91287-5_54

A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase

D Highton, D Chitnis, S Brigadoi, P Phan, I Tachtsidis, R Cooper, N Everdell, J Hebden, M Smith, C E Elwell

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Measurement of the oxidation state of cytochrome c oxidase (oxCCO) can inform directly on neuronal metabolism. Conventionally this has been measured in vivo using benchtop broadband near infrared spectroscopy (NIRS) systems. Spatially resolved measures of oxCCO have recently been made possible using a multichannel fibre-based broadband NIRS system. We describe the use of a fibreless multiwavelength NIRS system using light emitting diodes (LED) designed specifically to image localised changes in oxCCO and hence neuronal metabolism. A fibreless system consisting of four modules, each containing two LED sources and four photodiode detectors, was developed. Each LED source contained eight LED dies (780, 811, 818, 842, 850, 882, 891 and 901 nm) assembled in an area of 1.5 × 1.5 mm. A well-established hyperoxia protocol was used to evaluate the oxCCO spatially resolved measurement capabilities of the system and, subsequently, its imaging capabilities were tested using a functional activation paradigm. A multi-spectral image reconstruction approach was used to provide images of Δ[HbO2], Δ[HHb] and Δ[oxCCO] from the multi-distance, multi-channel optical datasets. This novel fibreless multiwavelength NIRS system allows imaging of localised changes in oxCCO in the human brain, and has potential for development as an inexpensive, wearable, continuous monitor of cerebral energetics in a range of experimental and clinical scenarios.

Original language	English
Title of host publication	Oxygen Transport to Tissue XL
Editors	Oliver Thews, Joseph C. LaManna, David K. Harrison
Pages	339-343
Number of pages	5
Volume	1072
ISBN (Electronic)	978-3-319-91287-5
DOIs	https://doi.org/10.1007/978-3-319-91287-5_54
Publication status	Published - 2018

Publication series

Name	Advances in Experimental Medicine and Biology
Publisher	Springer New York
ISSN (Print)	0065-2598

Keywords / Materials (for Non-textual outputs)

Brain/diagnostic imaging
Brain Mapping/instrumentation
Electron Transport Complex IV/analysis
Humans
Image Processing, Computer-Assisted/methods
Neurons/metabolism
Spectroscopy, Near-Infrared/instrumentation

Access to Document

10.1007/978-3-319-91287-5_54

Cite this

Highton, D., Chitnis, D., Brigadoi, S., Phan, P., Tachtsidis, I., Cooper, R., Everdell, N., Hebden, J., Smith, M., & Elwell, C. E. (2018). A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase. In O. Thews, J. C. LaManna, & D. K. Harrison (Eds.), Oxygen Transport to Tissue XL (Vol. 1072, pp. 339-343). (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-3-319-91287-5_54

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title = "A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase",

abstract = "Measurement of the oxidation state of cytochrome c oxidase (oxCCO) can inform directly on neuronal metabolism. Conventionally this has been measured in vivo using benchtop broadband near infrared spectroscopy (NIRS) systems. Spatially resolved measures of oxCCO have recently been made possible using a multichannel fibre-based broadband NIRS system. We describe the use of a fibreless multiwavelength NIRS system using light emitting diodes (LED) designed specifically to image localised changes in oxCCO and hence neuronal metabolism. A fibreless system consisting of four modules, each containing two LED sources and four photodiode detectors, was developed. Each LED source contained eight LED dies (780, 811, 818, 842, 850, 882, 891 and 901 nm) assembled in an area of 1.5 × 1.5 mm. A well-established hyperoxia protocol was used to evaluate the oxCCO spatially resolved measurement capabilities of the system and, subsequently, its imaging capabilities were tested using a functional activation paradigm. A multi-spectral image reconstruction approach was used to provide images of Δ[HbO2], Δ[HHb] and Δ[oxCCO] from the multi-distance, multi-channel optical datasets. This novel fibreless multiwavelength NIRS system allows imaging of localised changes in oxCCO in the human brain, and has potential for development as an inexpensive, wearable, continuous monitor of cerebral energetics in a range of experimental and clinical scenarios.",

keywords = "Brain/diagnostic imaging, Brain Mapping/instrumentation, Electron Transport Complex IV/analysis, Humans, Image Processing, Computer-Assisted/methods, Neurons/metabolism, Spectroscopy, Near-Infrared/instrumentation",

author = "D Highton and D Chitnis and S Brigadoi and P Phan and I Tachtsidis and R Cooper and N Everdell and J Hebden and M Smith and Elwell, {C E}",

year = "2018",

doi = "10.1007/978-3-319-91287-5_54",

language = "English",

isbn = "978-3-319-91285-1",

volume = "1072",

series = "Advances in Experimental Medicine and Biology",

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pages = "339--343",

editor = "Oliver Thews and LaManna, {Joseph C.} and Harrison, {David K.}",

booktitle = "Oxygen Transport to Tissue XL",

}

Highton, D, Chitnis, D, Brigadoi, S, Phan, P, Tachtsidis, I, Cooper, R, Everdell, N, Hebden, J, Smith, M & Elwell, CE 2018, A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase. in O Thews, JC LaManna & DK Harrison (eds), Oxygen Transport to Tissue XL. vol. 1072, Advances in Experimental Medicine and Biology, pp. 339-343. https://doi.org/10.1007/978-3-319-91287-5_54

A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase. / Highton, D; Chitnis, D; Brigadoi, S et al.
Oxygen Transport to Tissue XL. ed. / Oliver Thews; Joseph C. LaManna; David K. Harrison. Vol. 1072 2018. p. 339-343 (Advances in Experimental Medicine and Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase

AU - Highton, D

AU - Chitnis, D

AU - Brigadoi, S

AU - Phan, P

AU - Tachtsidis, I

AU - Cooper, R

AU - Everdell, N

AU - Hebden, J

AU - Smith, M

AU - Elwell, C E

PY - 2018

Y1 - 2018

N2 - Measurement of the oxidation state of cytochrome c oxidase (oxCCO) can inform directly on neuronal metabolism. Conventionally this has been measured in vivo using benchtop broadband near infrared spectroscopy (NIRS) systems. Spatially resolved measures of oxCCO have recently been made possible using a multichannel fibre-based broadband NIRS system. We describe the use of a fibreless multiwavelength NIRS system using light emitting diodes (LED) designed specifically to image localised changes in oxCCO and hence neuronal metabolism. A fibreless system consisting of four modules, each containing two LED sources and four photodiode detectors, was developed. Each LED source contained eight LED dies (780, 811, 818, 842, 850, 882, 891 and 901 nm) assembled in an area of 1.5 × 1.5 mm. A well-established hyperoxia protocol was used to evaluate the oxCCO spatially resolved measurement capabilities of the system and, subsequently, its imaging capabilities were tested using a functional activation paradigm. A multi-spectral image reconstruction approach was used to provide images of Δ[HbO2], Δ[HHb] and Δ[oxCCO] from the multi-distance, multi-channel optical datasets. This novel fibreless multiwavelength NIRS system allows imaging of localised changes in oxCCO in the human brain, and has potential for development as an inexpensive, wearable, continuous monitor of cerebral energetics in a range of experimental and clinical scenarios.

AB - Measurement of the oxidation state of cytochrome c oxidase (oxCCO) can inform directly on neuronal metabolism. Conventionally this has been measured in vivo using benchtop broadband near infrared spectroscopy (NIRS) systems. Spatially resolved measures of oxCCO have recently been made possible using a multichannel fibre-based broadband NIRS system. We describe the use of a fibreless multiwavelength NIRS system using light emitting diodes (LED) designed specifically to image localised changes in oxCCO and hence neuronal metabolism. A fibreless system consisting of four modules, each containing two LED sources and four photodiode detectors, was developed. Each LED source contained eight LED dies (780, 811, 818, 842, 850, 882, 891 and 901 nm) assembled in an area of 1.5 × 1.5 mm. A well-established hyperoxia protocol was used to evaluate the oxCCO spatially resolved measurement capabilities of the system and, subsequently, its imaging capabilities were tested using a functional activation paradigm. A multi-spectral image reconstruction approach was used to provide images of Δ[HbO2], Δ[HHb] and Δ[oxCCO] from the multi-distance, multi-channel optical datasets. This novel fibreless multiwavelength NIRS system allows imaging of localised changes in oxCCO in the human brain, and has potential for development as an inexpensive, wearable, continuous monitor of cerebral energetics in a range of experimental and clinical scenarios.

KW - Brain/diagnostic imaging

KW - Brain Mapping/instrumentation

KW - Electron Transport Complex IV/analysis

KW - Humans

KW - Image Processing, Computer-Assisted/methods

KW - Neurons/metabolism

KW - Spectroscopy, Near-Infrared/instrumentation

U2 - 10.1007/978-3-319-91287-5_54

DO - 10.1007/978-3-319-91287-5_54

M3 - Chapter (peer-reviewed)

C2 - 30178368

SN - 978-3-319-91285-1

VL - 1072

T3 - Advances in Experimental Medicine and Biology

SP - 339

EP - 343

BT - Oxygen Transport to Tissue XL

A2 - Thews, Oliver

A2 - LaManna, Joseph C.

A2 - Harrison, David K.

ER -

Highton D, Chitnis D, Brigadoi S, Phan P, Tachtsidis I, Cooper R et al. A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase. In Thews O, LaManna JC, Harrison DK, editors, Oxygen Transport to Tissue XL. Vol. 1072. 2018. p. 339-343. (Advances in Experimental Medicine and Biology). Epub 2018 Aug 30. doi: 10.1007/978-3-319-91287-5_54

A Fibreless Multiwavelength NIRS System for Imaging Localised Changes in Cerebral Oxidised Cytochrome C Oxidase

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Keywords / Materials (for Non-textual outputs)

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