A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R Rios, Gloria Garoffolo, Giulia Rinaldi, Alicia Megia-fernandez, Silvia Ferrari, Calum T. Robb, Adriano G. Rossi, Maurizio Pesce, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract

A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.
Original languageEnglish
Pages (from-to)97-100
JournalChemical Communications
Volume57
Issue number1
DOIs
Publication statusPublished - 8 Dec 2020

Fingerprint Dive into the research topics of 'A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation'. Together they form a unique fingerprint.

Cite this