A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R Rios; Gloria Garoffolo; Giulia Rinaldi; Alicia Megia-fernandez; Silvia Ferrari; Calum T. Robb; Adriano G. Rossi; Maurizio Pesce; Mark Bradley

doi:10.1039/D0CC07028A

A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R Rios, Gloria Garoffolo, Giulia Rinaldi, Alicia Megia-fernandez, Silvia Ferrari, Calum T. Robb, Adriano G. Rossi, Maurizio Pesce, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.

Original language	English
Pages (from-to)	97-100
Journal	Chemical Communications
Volume	57
Issue number	1
DOIs	https://doi.org/10.1039/D0CC07028A
Publication status	Published - 8 Dec 2020

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10.1039/D0CC07028A

d0cc07028a
This article is Open Access.
Final published version, 3.92 MBLicence: Creative Commons: Attribution Non-Commercial (CC-BY-NC)

http://xlink.rsc.org/?DOI=D0CC07028A

The role of cyclin-dependent kinase-9 inhibition in promoting the resolution of chronic inflammation
Rossi, A. & Haslett, C.
MRC
1/05/13 → 30/10/19
Project: Research

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title = "A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation",

abstract = "A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.",

author = "Rios, {Maria R} and Gloria Garoffolo and Giulia Rinaldi and Alicia Megia-fernandez and Silvia Ferrari and Robb, {Calum T.} and Rossi, {Adriano G.} and Maurizio Pesce and Mark Bradley",

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AU - Garoffolo, Gloria

AU - Rinaldi, Giulia

AU - Megia-fernandez, Alicia

AU - Ferrari, Silvia

AU - Robb, Calum T.

AU - Rossi, Adriano G.

AU - Pesce, Maurizio

AU - Bradley, Mark

PY - 2020/12/8

Y1 - 2020/12/8

N2 - A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.

AB - A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.

U2 - 10.1039/D0CC07028A

DO - 10.1039/D0CC07028A

M3 - Article

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VL - 57

SP - 97

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JO - Chemical Communications

JF - Chemical Communications

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A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

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The role of cyclin-dependent kinase-9 inhibition in promoting the resolution of chronic inflammation

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