A genome wide association analysis identifying SNPs for PRRS tolerance on a commercial pig farm

C.R.G. Lewis, M. Torremorell, L. Galina-Pantoja, N. Deeb, M.A. Mellencamp, A.L. Archibald, S.C. Bishop

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is currently the most economically important viral disease affecting pig production outside Australia. This study utilised commercial data to perform a genome wide association study looking for single nucleotide polymorphism (SNP) markers associated with PRRS resistance or tolerance, as assessed indirectly from reproductive traits. In total, phenotypes were measured on 1,545 sows, with the data split according to whether the trait was measured during a healthy PRRS-free phase on the farm (4,378 litters from 1,019 sows) or a diseased phase (1,977 litters from 1,526 sows). All animals were genotyped using the Illumina porcine 7k SNP chip. Associations between each individual SNP and reproductive outcomes were assessed using the residual values from a linear mixed (animal) model analysis of each trait in a series of single SNP analyses. Significant SNP associations were only observed for reproductive traits recorded during the disease phase, implying specificity of identified SNPs to a PRRS active phase. After correction for false positives, six significant SNP markers were identified for piglets born alive, piglets born dead and mummified piglets born per litter. SNP effects were then re-estimated from linear mixed model analyses of the data in which the significant SNPs were fitted as additional fixed effects. The SNPs are generally additive in their mode of action and on average the significant SNPs explain 4.7% of the genetic variation for born alive, 1.6% for born dead and 2.2% for born mummified. After further validation and research, these SNPs may allow breeding of pigs that are more robust in the face of PRRSV infection.
Original languageUndefined/Unknown
Title of host publicationAssociation for the advancement of animal breeding and genetics. Proceedings of the 18th conference, Barossa Valley, South Australia, 28 September - 1 October 2009
Place of PublicationAdelaide
PublisherAssociation for the Advancement of Animal Breeding and Genetics
Pages187-190
Number of pages4
Publication statusPublished - 2009
EventAssociation for the Advancement of Animal Breeding and Genetics - Barossa Valley, Australia
Duration: 28 Sep 20091 Oct 2009

Conference

ConferenceAssociation for the Advancement of Animal Breeding and Genetics
Country/TerritoryAustralia
CityBarossa Valley
Period28/09/091/10/09

Keywords

  • disease genetics Pigs SNP virus

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