A hemoglobin with an optical function

A H Burr, P Hunt, D R Wagar, S Dewilde, M L Blaxter, J R Vanfleteren, L Moens, Mark Blaxter

Research output: Contribution to journalArticlepeer-review

Abstract

Hemoglobins are best known as oxygen transport proteins. Here we describe a hemoglobin from the parasitic nematode Mermis nigrescens (Mn-GLB-E) that has an optical, light shadowing function. The protein accumulates to high concentration as intracellular crystals in the ocellus of mature phototactic adult females while also being expressed at low concentration in other tissues. It differs in sequence and expression pattern from Mn-GLB-B, a second Mermis globin. It retains the structure and oxygen-binding and light-absorbing properties typical of nematode hemoglobins. As such, recruitment to a shadowing role in the eye appears to have occurred by changes in expression without modification of biochemistry. Both globins are coded by genes interrupted by two introns at the conserved positions B12.2 and G7.0, which is in agreement with the 3exon/2intron pattern model of globin gene evolution.
Original languageEnglish
Pages (from-to)4810-4815
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number7
Publication statusPublished - 2000

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers
  • DNA, Complementary
  • Eye
  • Female
  • Hemoglobins
  • Mermithoidea
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Vision, Ocular

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