TY - JOUR
T1 - A high-fidelity microfluidic platform reveals retrograde propagation as the main mechanism of α-Synuclein spread in human neurons
AU - Vroman, Rozan
AU - de Lichtervelde, Lorenzo
AU - Singh Dolt, Karamjit
AU - Robertson, Graham
AU - Kriek, Marco
AU - Barbato, Michela
AU - Cholewa-Waclaw, Justyna
AU - Kunath, Tilo
AU - Downey, Patrick
AU - Zagnoni, Michele
N1 - We would like to dedicate this paper to the memory of Terry Baker.
PY - 2025/4/20
Y1 - 2025/4/20
N2 - α-Synuclein (αSyn) is a major component of Lewy bodies and Lewy neurites, which are a pathological hallmark of Parkinson’s disease (PD). Pathologically aggregated forms of αSyn can spread along neurites and induce the misfolding of normal αSyn. To elucidate how αSyn pathology propagates between brain areas, we developed a novel in vitro microfluidic platform to study the intracellular transport of preformed fibrils and the induction and spread of αSyn aggregates. Patient-derived midbrain dopaminergic (mDA) neurons were cultured in microfluidic devices designed to maintain unidirectional axonal connections between fluidically isolated mDA neuronal cultures for over 3 months. Using αSyn preformed fibrils to induce Lewy-like pathology, we found that anterograde spread of αSyn fibrils was slow and occurred at low levels, while retrograde spread was significantly more efficient. This is in line with observations in animal models and shows that the platform provides an innovative new tool for studying PD in vitro.
AB - α-Synuclein (αSyn) is a major component of Lewy bodies and Lewy neurites, which are a pathological hallmark of Parkinson’s disease (PD). Pathologically aggregated forms of αSyn can spread along neurites and induce the misfolding of normal αSyn. To elucidate how αSyn pathology propagates between brain areas, we developed a novel in vitro microfluidic platform to study the intracellular transport of preformed fibrils and the induction and spread of αSyn aggregates. Patient-derived midbrain dopaminergic (mDA) neurons were cultured in microfluidic devices designed to maintain unidirectional axonal connections between fluidically isolated mDA neuronal cultures for over 3 months. Using αSyn preformed fibrils to induce Lewy-like pathology, we found that anterograde spread of αSyn fibrils was slow and occurred at low levels, while retrograde spread was significantly more efficient. This is in line with observations in animal models and shows that the platform provides an innovative new tool for studying PD in vitro.
U2 - 10.1038/s41531-025-00936-x
DO - 10.1038/s41531-025-00936-x
M3 - Article
SN - 2373-8057
VL - 11
JO - npj Parkinson's Disease
JF - npj Parkinson's Disease
IS - 1
M1 - 80
ER -