Abstract / Description of output
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.
Original language | English |
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Pages (from-to) | 85-93 |
Number of pages | 9 |
Journal | Journal of Immunological Methods |
Volume | 239 |
Issue number | 1-2 |
Publication status | Published - 26 May 2000 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Antigens, Protozoan/immunology
- CD4-Positive T-Lymphocytes
- Cattle
- Cell Division
- Cell Line
- Ehrlichia ruminantium/immunology
- Endothelium, Vascular/cytology
- Histocompatibility Antigens Class I/immunology
- Histocompatibility Antigens Class II/biosynthesis
- Interferon-gamma/pharmacology
- Iodine Radioisotopes
- Lymphocyte Activation/immunology
- Recombinant Proteins
- Sensitivity and Specificity
- T-Lymphocytes, Cytotoxic/cytology
- T-Lymphocytes, Cytotoxic/immunology
- Theileria parva/immunology