A highly sensitive, non-radioactive assay for T cell activation in cattle: applications in screening for antigens recognised by CD4(+) and CD8(+) T cells

Keith Ballingall, Duncan M. Mwangi, Niall MacHugh, Evans L. N. Taracha, Philippe Totte, Declan McKeever

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.
Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalJournal of Immunological Methods
Volume239
Issue number1-2
Publication statusPublished - 26 May 2000

Keywords

  • Animals
  • Antigens, Protozoan/immunology
  • CD4-Positive T-Lymphocytes
  • Cattle
  • Cell Division
  • Cell Line
  • Ehrlichia ruminantium/immunology
  • Endothelium, Vascular/cytology
  • Histocompatibility Antigens Class I/immunology
  • Histocompatibility Antigens Class II/biosynthesis
  • Interferon-gamma/pharmacology
  • Iodine Radioisotopes
  • Lymphocyte Activation/immunology
  • Recombinant Proteins
  • Sensitivity and Specificity
  • T-Lymphocytes, Cytotoxic/cytology
  • T-Lymphocytes, Cytotoxic/immunology
  • Theileria parva/immunology

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