A Key Role for Membrane Transporter NKCC1 in Mediating Chondrocyte Volume Increase in the Mammalian Growth Plate

P. G. Bush, M. Pritchard, M. Y. Loqman, T. A. Damron, A. C. Hall

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms that underlie growth plate chondrocyte volume increase and hence bone lengthening are poorly understood. Many cell types activate the Na-K-Cl cotransporter (NKCC) to bring about volume increase. We hypothesised that NKCC may be responsible for the volume expansion of hypertrophic chondrocytes. Metatarsals/metacarpals from 16 rat pups (P-7) were incubated in the presence/absence of the specific NKCC inhibitor bumetanide and measurement of whole-bone lengths and histologic analysis of the growth plate were done after 24 hours. Fluorescent NKCC immunohistochemistry was visualised using a confocal laser scanning microscopy on seven rat tibial growth plates (P-7). Microarray analysis was performed on mRNA isolated from proliferative and hypertrophic zone cells of tibial growth plates from five rats of each of three ages (P-49/53/58). Exposure to bumetanide resulted in approximately 35% reduction (paired Student's t test, p
Original languageEnglish
Pages (from-to)1594-1603
Number of pages10
JournalJournal of Bone and Mineral Research
Volume25
Issue number7
DOIs
Publication statusPublished - Jul 2010

Keywords

  • growth plate
  • hypertrophic chondrocytes
  • NKCC
  • volume
  • rat

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