[A kinetic model of beta-galactosidase of Escherichia coli]

EA Metelkin, GV Lebedeva, Igor Goryanin, OV Demin

Research output: Contribution to journalArticlepeer-review

Abstract

On the basis of previously published experimental data, the catalytic cycle of Escherichia coli galactosidase has been reconstructed. The enzyme plays a key role in the transcription regulation of lac operon. Using the proposed scheme, we have built a kinetic model that takes into account the reactions of hydrolysis, isomerization as well as galactosylation. Using the experimental data and optimization algorithms, all parameters of the model have been estimated. On the basis of the model, the features of beta-galactosidase functioning have been predicted in a wide range of conditions.
Translated title of the contribution[A kinetic model of beta-galactosidase of Escherichia coli]
Original languageOther
Pages (from-to)226-234
Number of pages9
JournalBiofizika
Volume54
Issue number2
Publication statusPublished - 2008

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