A kinetic model of Escherichia coli β-galactosidase

E.A. Metelkin, G.V. Lebedeva, I.I. Goryanin, O.V. Demin

Research output: Contribution to journalArticlepeer-review

Abstract

On the basis of published experimental data, we have reconstructed the catalytic cycle of Escherichia coli β-galactosidase, a key enzyme in transcription regulation of the lac operon. A kinetic model thus built takes into account the reactions of hydrolysis and isomerization as well as galactosylation in the catalytic site. Using the experimental data and numerical optimization algorithms, all parameters of the model have been determined. With the model, the specific features of β-galactosidase behavior are predicted in a wide range of conditions.
Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalBiophysics
Volume54
Issue number2
DOIs
Publication statusPublished - 2009

Keywords

  • β-galactosidase
  • lactose
  • kinetic modeling

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