A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

Ahsan R. Akram, Nicolaos Avlonitis, Annamaria Lilienkampf, Ana M. Perez-Lopez, Neil McDonald, Sunay V. Chankeshwara, Emma Scholefield, Christopher Haslett, Mark Bradley, Kevin Dhaliwal

Research output: Contribution to journalArticlepeer-review

Abstract

The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, Ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.
Original languageEnglish
Pages (from-to)6971-6979
Number of pages9
JournalChemical Science
Volume6
Issue number12
Early online date29 Jun 2015
DOIs
Publication statusPublished - 1 Dec 2015

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