A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

Ahsan R. Akram; Nicolaos Avlonitis; Annamaria Lilienkampf; Ana M. Perez-Lopez; Neil McDonald; Sunay V. Chankeshwara; Emma Scholefield; Christopher Haslett; Mark Bradley; Kevin Dhaliwal

doi:10.1039/C5SC00960J

A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

Ahsan R. Akram, Nicolaos Avlonitis, Annamaria Lilienkampf, Ana M. Perez-Lopez, Neil McDonald, Sunay V. Chankeshwara, Emma Scholefield, Christopher Haslett, Mark Bradley, Kevin Dhaliwal

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Abstract / Description of output

The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, Ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.

Original language	English
Pages (from-to)	6971-6979
Number of pages	9
Journal	Chemical Science
Volume	6
Issue number	12
Early online date	29 Jun 2015
DOIs	https://doi.org/10.1039/C5SC00960J
Publication status	Published - 1 Dec 2015

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Ahsan Akram
- Deanery of Clinical Sciences - UKRI Future Leaders Fellow and Clinician Scientist
- Edinburgh Cancer Research Centre - Cancer Research UK Clinician Scientist
- Centre for Inflammation Research
Person: Academic: Research Active (Research Assistant)
Kevin Dhaliwal
- Kev.Dhaliwaled.acuk
- Deanery of Clinical Sciences - Personal Chair of Molecular Imaging & Healthcare
- Centre for Inflammation Research
- Edinburgh Imaging
Person: Academic: Research Active , Academic: Research Active (Research Assistant)
Annamaria Lilienkampf
- School of Chemistry - Senior Lecturer
- EaStCHEM
Person: Academic: Research Active

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Akram, A. R., Avlonitis, N., Lilienkampf, A., Perez-Lopez, A. M., McDonald, N., Chankeshwara, S. V., Scholefield, E., Haslett, C., Bradley, M., & Dhaliwal, K. (2015). A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue. Chemical Science, 6(12), 6971-6979. https://doi.org/10.1039/C5SC00960J

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title = "A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue",

abstract = "The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, Ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.",

author = "Akram, {Ahsan R.} and Nicolaos Avlonitis and Annamaria Lilienkampf and Perez-Lopez, {Ana M.} and Neil McDonald and Chankeshwara, {Sunay V.} and Emma Scholefield and Christopher Haslett and Mark Bradley and Kevin Dhaliwal",

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doi = "10.1039/C5SC00960J",

language = "English",

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T1 - A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

AU - Akram, Ahsan R.

AU - Avlonitis, Nicolaos

AU - Lilienkampf, Annamaria

AU - Perez-Lopez, Ana M.

AU - McDonald, Neil

AU - Chankeshwara, Sunay V.

AU - Scholefield, Emma

AU - Haslett, Christopher

AU - Bradley, Mark

AU - Dhaliwal, Kevin

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, Ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.

AB - The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, Ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.

U2 - 10.1039/C5SC00960J

DO - 10.1039/C5SC00960J

M3 - Article

SN - 2041-6539

VL - 6

SP - 6971

EP - 6979

JO - Chemical Science

JF - Chemical Science

IS - 12

ER -

A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

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Ahsan Akram

Kevin Dhaliwal

Annamaria Lilienkampf

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