A landmark-free morphometric staging system for the mouse limb bud

Bernd Boehm, Michael Rautschka, Laura Quintana, Jelena Raspopovic, Ziga Jan, James Sharpe

Research output: Contribution to journalArticlepeer-review

Abstract

We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about ±2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns.
Original languageEnglish
Pages (from-to)1227-34
Number of pages8
JournalDevelopment
Volume138
Issue number6
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Animals
  • Body Weights and Measures
  • Embryonic Development
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gestational Age
  • Growth Charts
  • Limb Buds
  • Mice
  • Models, Biological
  • Organ Size
  • Reproducibility of Results
  • Research Design
  • SOX9 Transcription Factor
  • Time Factors

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