A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

Kathryn S. Potts; Tobias J. Sargeant; John F. Markham; Wei Shi; Christine Biben; Emma C. Josefsson; Lachlan W. Whitehead; Kelly L. Rogers; Anna Liakhovitskaia; Gordon K. Smyth; Benjamin T. Kile; Alexander Medvinsky; Warren S. Alexander; Douglas J. Hilton; Samir Taoudi

doi:10.1182/blood-2014-02-559468

A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

Kathryn S. Potts, Tobias J. Sargeant, John F. Markham, Wei Shi, Christine Biben, Emma C. Josefsson, Lachlan W. Whitehead, Kelly L. Rogers, Anna Liakhovitskaia, Gordon K. Smyth, Benjamin T. Kile, Alexander Medvinsky, Warren S. Alexander, Douglas J. Hilton, Samir Taoudi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

Original language	English
Pages (from-to)	2725-2729
Number of pages	5
Journal	Blood
Volume	124
Issue number	17
DOIs	https://doi.org/10.1182/blood-2014-02-559468
Publication status	Published - 23 Oct 2014

Keywords / Materials (for Non-textual outputs)

blood platelets
diploidy
embryo
meckel-gruber syndrome
megakaryocytes
mice
polyploidy
proplatelet
stem cells
hematopoietic stem cells

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10.1182/blood-2014-02-559468

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Potts, K. S., Sargeant, T. J., Markham, J. F., Shi, W., Biben, C., Josefsson, E. C., Whitehead, L. W., Rogers, K. L., Liakhovitskaia, A., Smyth, G. K., Kile, B. T., Medvinsky, A., Alexander, W. S., Hilton, D. J., & Taoudi, S. (2014). A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. Blood, 124(17), 2725-2729. https://doi.org/10.1182/blood-2014-02-559468

@article{a7f9ad152f984cf7995ca7aafa89e540,

title = "A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo",

abstract = "In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.",

keywords = "blood platelets, diploidy, embryo, meckel-gruber syndrome, megakaryocytes, mice, polyploidy, proplatelet, stem cells, hematopoietic stem cells",

author = "Potts, {Kathryn S.} and Sargeant, {Tobias J.} and Markham, {John F.} and Wei Shi and Christine Biben and Josefsson, {Emma C.} and Whitehead, {Lachlan W.} and Rogers, {Kelly L.} and Anna Liakhovitskaia and Smyth, {Gordon K.} and Kile, {Benjamin T.} and Alexander Medvinsky and Alexander, {Warren S.} and Hilton, {Douglas J.} and Samir Taoudi",

year = "2014",

month = oct,

day = "23",

doi = "10.1182/blood-2014-02-559468",

language = "English",

volume = "124",

pages = "2725--2729",

journal = "Blood",

issn = "0006-4971",

publisher = "American Society of Hematology",

number = "17",

}

Potts, KS, Sargeant, TJ, Markham, JF, Shi, W, Biben, C, Josefsson, EC, Whitehead, LW, Rogers, KL, Liakhovitskaia, A, Smyth, GK, Kile, BT, Medvinsky, A, Alexander, WS, Hilton, DJ & Taoudi, S 2014, 'A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo', Blood, vol. 124, no. 17, pp. 2725-2729. https://doi.org/10.1182/blood-2014-02-559468

TY - JOUR

T1 - A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

AU - Potts, Kathryn S.

AU - Sargeant, Tobias J.

AU - Markham, John F.

AU - Shi, Wei

AU - Biben, Christine

AU - Josefsson, Emma C.

AU - Whitehead, Lachlan W.

AU - Rogers, Kelly L.

AU - Liakhovitskaia, Anna

AU - Smyth, Gordon K.

AU - Kile, Benjamin T.

AU - Medvinsky, Alexander

AU - Alexander, Warren S.

AU - Hilton, Douglas J.

AU - Taoudi, Samir

PY - 2014/10/23

Y1 - 2014/10/23

N2 - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

AB - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

KW - blood platelets

KW - diploidy

KW - embryo

KW - meckel-gruber syndrome

KW - megakaryocytes

KW - mice

KW - polyploidy

KW - proplatelet

KW - stem cells

KW - hematopoietic stem cells

U2 - 10.1182/blood-2014-02-559468

DO - 10.1182/blood-2014-02-559468

M3 - Article

C2 - 25079356

AN - SCOPUS:84908242967

SN - 0006-4971

VL - 124

SP - 2725

EP - 2729

JO - Blood

JF - Blood

IS - 17

ER -

A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

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Keywords / Materials (for Non-textual outputs)

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