A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

Kathryn S. Potts, Tobias J. Sargeant, John F. Markham, Wei Shi, Christine Biben, Emma C. Josefsson, Lachlan W. Whitehead, Kelly L. Rogers, Anna Liakhovitskaia, Gordon K. Smyth, Benjamin T. Kile, Alexander Medvinsky, Warren S. Alexander, Douglas J. Hilton, Samir Taoudi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

Original languageEnglish
Pages (from-to)2725-2729
Number of pages5
JournalBlood
Volume124
Issue number17
DOIs
Publication statusPublished - 23 Oct 2014

Keywords

  • blood platelets
  • diploidy
  • embryo
  • meckel-gruber syndrome
  • megakaryocytes
  • mice
  • polyploidy
  • proplatelet
  • stem cells
  • hematopoietic stem cells

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