A link between mRNA turnover and RNA interference in Arabidopsis

S Gazzani, T Lawrenson, C Woodward, D Headon, R Sablowski

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In RNA interference (RNAi), double-stranded RNA (dsRNA) triggers degradation of homologous messenger RNA. In many organisms, RNA-dependent RNA polymerase (RdRp) is required to initiate or amplify RNAi, but the substrate for dsRNA synthesis in vivo is not known. Here, we show that RdRp-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4, which is a ribonuclease (RNase) implicated in mRNA turnover by means of decapping and 5'-3' exonucleolysis. When both XRN4 and the RdRp were mutated, the plants accumulated decapped transgene mRNA. We propose that mRNAs lacking a cap structure become exposed to RdRp to initiate or maintain RNAi.
Original languageEnglish
Pages (from-to)1046-8
Number of pages3
Issue number5698
Publication statusPublished - 5 Nov 2004

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Arabidopsis
  • Arabidopsis Proteins
  • Exoribonucleases
  • Gene Silencing
  • Homeodomain Proteins
  • Mutation
  • Plant Proteins
  • Plants, Genetically Modified
  • RNA Caps
  • RNA Interference
  • RNA Replicase
  • RNA, Messenger
  • RNA, Plant
  • Rats
  • Recombinant Fusion Proteins


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