A metabolomics-based approach to identify lineage guiding molecules in Pericyte cultures

Enateri V. Alakpa, Christopher C. West, Laura Goldie, Mhairi M. Harper, Karl Burgess, Rein V. Ulijn, Matthew J. Dalby

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We report the use of self-assembled peptide (F2/S) hydrogels and cellular metabolomics to identify a number of innate molecules that are integral to the metabolic processes which drive cellular differentiation of multipotent pericyte stem cells. The culture system relies solely on substrate mechanics to induce differentiation in the absence of traditional differentiation media and therefore is a non-invasive approach to assessing cellular behavior at the molecular level and identifying key metabolites in this process. This novel approach demonstrates that simple metabolites can provide an alternative means to direct stem cell differentiation and that biomaterials can be used to identify them simply and quickly.
Original languageEnglish
Title of host publicationPericytes
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Chapter5
Pages47-59
Number of pages13
Volume2235
ISBN (Electronic)978-1-0716-1056-5
ISBN (Print)978-1-0716-1055-8
DOIs
Publication statusPublished - 13 Feb 2021

Publication series

NamePericytes
Volume2235
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • differentiation
  • pericytes
  • hydrogels
  • biomaterials
  • metabolomics

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