A Method for Positive and Negative Selection of Plasmodium falciparum Platelet-Mediated Clumping Parasites and Investigation of the Role of CD36

Mònica Arman, Yvonne Adams, Gabriella Lindergard, J Alexandra Rowe

Research output: Contribution to journalArticlepeer-review

Abstract

Platelet-mediated clumping of Plasmodium falciparum infected erythrocytes (IEs) is a frequently observed parasite adhesion phenotype. The importance of clumping in severe malaria and the molecular mechanisms behind this phenomenon are incompletely understood. Three platelet surface molecules have previously been identified as clumping receptors: CD36, globular C1q receptor (gC1qR/HABP1/p32), and P-selectin (CD62P), but the parasite ligands mediating this phenotype are unknown. The aim of this work was to develop a selection method to facilitate investigations of the molecular mechanisms of clumping in selected P. falciparum lines. Magnetic beads coated with anti-platelet antibodies were used to positively and negatively select clumping IEs from P. falciparum strains IT, HB3, 3D7 and Dd2. Clumping in all four positively selected parasite lines was abolished by antibodies to CD36, but was not affected by antibodies to gC1qR or P-selectin. Clumping positive lines showed significantly higher binding to CD36 than clumping negative lines in flow adhesion assays (strains IT, HB3 and 3D7, p
Original languageEnglish
Pages (from-to)e55453
JournalPLoS ONE
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 2013

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