A micro electrical impedance tomography (EIT) sensor with radially distributed planar electrodes was designed, characterized, and experimentally validated for real-time and non-destructive 3-D cultivated cell imaging. The 12-mm cylindrical sensor was consisted of 17 circular micro electrodes, including 16 sensing electrodes and a reference electrode. The sensor's characteristics, i.e., dynamic range of measurement and sensitivity, were examined by finite element simulations and actual measurements. MCF-7 breast cancer cells were then cultivated to form the spherical aggregate and 3-D image reconstructions were performed on three solutions containing varying number and locations of cell aggregates. Furthermore, real-time imaging of cell-drug response between a MCF-7 cell aggregate and diluted Triton X-100 solution was carried out and the 3-D conductivity variation associated with this transient process was captured successfully and reconstructed in high accuracy. The simulation, 3-D cell aggregate imaging experiments and real-time reconstructions of cell-drug response suggest that the EIT-based micro sensor has the potential to study non-destructively and in real time the dynamic biological behavior of a 3-D cell culture system.
|Pages (from-to)||5402 - 5412|
|Journal||IEEE Sensors Journal|
|Early online date||9 May 2018|
|Publication status||Published - 1 Jul 2018|
- 3-D cell imaging
- electrical impedance tomography
- image reconstruction
- micro sensor
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- School of Engineering - Chancellor's Fellow in Data Driven Innovation
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