A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

Lina Vasilauskaite; Dimitrios Vitsios; Rebecca V. Berrens; Claudia Carrieri; Wolf Reik; Anton J Enright; Donal O'Carroll

doi:10.1038/nsmb.3413

A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

Lina Vasilauskaite, Dimitrios Vitsios, Rebecca V. Berrens, Claudia Carrieri, Wolf Reik, Anton J Enright, Donal O'Carroll

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI–piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.

Original language	English
Pages (from-to)	604–606
Number of pages	3
Journal	Nature Structural & Molecular Biology
Volume	24
Issue number	7
Early online date	22 May 2017
DOIs	https://doi.org/10.1038/nsmb.3413
Publication status	Published - 1 Jul 2017

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OA-DO-Apr17-L.Vasiliauskaite_et.al.2017Accepted author manuscript, 14.1 MB

ncRNA: Non-coding RNA pathways and the mammalian male germline
O'Carroll, D.
EU government bodies
1/01/16 → 31/12/17
Project: Research

Donal O'Carroll
- School of Biological Sciences - Chair of Stem Cell Biology
- Centre for Regenerative Medicine
Person: Academic: Research Active

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title = "A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming",

abstract = "In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI–piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.",

author = "Lina Vasilauskaite and Dimitrios Vitsios and Berrens, {Rebecca V.} and Claudia Carrieri and Wolf Reik and Enright, {Anton J} and Donal O'Carroll",

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T1 - A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

AU - Vasilauskaite, Lina

AU - Vitsios, Dimitrios

AU - Berrens, Rebecca V.

AU - Carrieri, Claudia

AU - Reik, Wolf

AU - Enright, Anton J

AU - O'Carroll, Donal

PY - 2017/7/1

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N2 - In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI–piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.

AB - In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI–piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.

U2 - 10.1038/nsmb.3413

DO - 10.1038/nsmb.3413

M3 - Article

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EP - 606

JO - Nature Structural & Molecular Biology

JF - Nature Structural & Molecular Biology

IS - 7

ER -

A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

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ncRNA: Non-coding RNA pathways and the mammalian male germline

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Donal O'Carroll

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