A Miniature Electrical Impedance Tomography Sensor and 3-D Image Reconstruction for Cell Imaging

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Real-time quantitative imaging is becoming highly desirable to study nondestructively the biological behavior of 3-D cell culture systems. In this paper, we investigate the feasibility of quantitative imaging/monitoring of 3-D cell culture processes via electrical impedance tomography (EIT), which is capable of generating conductivity images in a non-destructive manner with high temporal resolution. To this end, a planar miniature EIT sensor amenable to standard cell culture format is designed, and a 3-D forward model for the sensor is developed for 3-D imaging. Furthermore, a novel 3-D-Laplacian and sparsity joint regularization algorithm is proposed for enhanced 3-D image reconstruction. Simulation phantoms with spheres at various vertical and horizontal positions were imaged for 3-D performance evaluation. In addition, experiments on human breast cancer cell spheroid and a triangular breast cancer cell pellet were carried out for experimental verification. The results have shown that the stable measurement on high conductive cell culture medium and the significant improvement of image quality based on the proposed regularization method are achieved. It demonstrates the feasibility of using the miniature EIT sensor and 3-D image reconstruction algorithm to visualize 3-D cell cultures, such as spheroids or artificial tissues and organs. The established work would expedite real-time quantitative imaging of 3-D cell culture for assessment of cellular dynamics.
Original languageEnglish
Pages (from-to)514-523
JournalIEEE Sensors Journal
Volume17
Issue number2
Early online date21 Nov 2016
DOIs
Publication statusPublished - 15 Jan 2017

Keywords / Materials (for Non-textual outputs)

  • Sensors
  • Three-dimensional displays
  • tomography
  • image sensors
  • Image Reconstruction
  • electrodes
  • Cancer
  • cellular biophysics
  • Electrical impedance imaging
  • medical image processing
  • Phantoms
  • Cell culture
  • Electrical impedance tomography
  • 3D image reconstruction
  • miniature sensor

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