Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.
|Journal||Transactions of the Royal Society of Tropical Medicine and Hygiene|
|Publication status||Published - 1 Nov 2018|
- bovine cysticercosis
- field diagnosis
- HP10 antigen ELISA
- HP10 antigen LFA
- neurocysticercosis (NCC)