A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives

R Michael E Parkhouse, Arturo Carpio, Alfredo Campoverde, Patricia Sastre, Glenda Rojas, Leslie J S Harrison, Maria Milagros Cortez

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.

Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.

Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.

Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.

Original languageEnglish
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • bovine cysticercosis
  • field diagnosis
  • HP10 antigen ELISA
  • HP10 antigen LFA
  • neurocysticercosis (NCC)

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