A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum

A Fenton; J A Sinclair; Gary Entrican; J A Herring; C Malloy; P F  Nettleton

doi:10.1016/0378-1135(91)90067-P

A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum

A Fenton, J A Sinclair, Gary Entrican, J A Herring, C Malloy, P F Nettleton

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibody to border disease virus (BDV) in sheep serum. A monoclonal antibody bound to 96-well microplates was used to capture antigen from detergent-solubilised BDV-infected cells. Single dilutions of test sera were then added to wells containing bound BDV antigen and control wells containing uninfected cell lysates. Specific antibody to BDV was detected by an anti-ovine IgG antiserum conjugated with horseradish peroxidase and the results expressed as ELISA units with reference to a standard curve. Sequential sera from 16 experimentally infected sheep and single sera from 103 sheep involved in a field outbreak were tested in the ELISA and for neutralising antibody. There was good qualitative correlation between the two tests.

Original language	English
Pages (from-to)	327-33
Number of pages	7
Journal	Veterinary Microbiology
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/0378-1135(91)90067-P
Publication status	Published - Aug 1991

Keywords / Materials (for Non-textual outputs)

animals
Antibodies, Monoclonal
Antibodies, Viral
Border Disease
Enzyme-Linked Immunosorbent Assay
horseradish peroxidase
Immunoglobulins
Neutralization Tests
pestvirus
sheep

Access to Document

10.1016/0378-1135(91)90067-P

Cite this

@article{1a6e10e27f76402db1e349bee8b9b2be,

title = "A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum",

abstract = "An enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibody to border disease virus (BDV) in sheep serum. A monoclonal antibody bound to 96-well microplates was used to capture antigen from detergent-solubilised BDV-infected cells. Single dilutions of test sera were then added to wells containing bound BDV antigen and control wells containing uninfected cell lysates. Specific antibody to BDV was detected by an anti-ovine IgG antiserum conjugated with horseradish peroxidase and the results expressed as ELISA units with reference to a standard curve. Sequential sera from 16 experimentally infected sheep and single sera from 103 sheep involved in a field outbreak were tested in the ELISA and for neutralising antibody. There was good qualitative correlation between the two tests.",

keywords = "animals, Antibodies, Monoclonal, Antibodies, Viral, Border Disease, Enzyme-Linked Immunosorbent Assay, horseradish peroxidase, Immunoglobulins, Neutralization Tests, pestvirus, sheep",

author = "A Fenton and Sinclair, \{J A\} and Gary Entrican and Herring, \{J A\} and C Malloy and Nettleton, \{P F\}",

year = "1991",

month = aug,

doi = "10.1016/0378-1135(91)90067-P",

language = "English",

volume = "28",

pages = "327--33",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "4",

}

TY - JOUR

T1 - A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum

AU - Fenton, A

AU - Sinclair, J A

AU - Entrican, Gary

AU - Herring, J A

AU - Malloy, C

AU - Nettleton, P F

PY - 1991/8

Y1 - 1991/8

N2 - An enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibody to border disease virus (BDV) in sheep serum. A monoclonal antibody bound to 96-well microplates was used to capture antigen from detergent-solubilised BDV-infected cells. Single dilutions of test sera were then added to wells containing bound BDV antigen and control wells containing uninfected cell lysates. Specific antibody to BDV was detected by an anti-ovine IgG antiserum conjugated with horseradish peroxidase and the results expressed as ELISA units with reference to a standard curve. Sequential sera from 16 experimentally infected sheep and single sera from 103 sheep involved in a field outbreak were tested in the ELISA and for neutralising antibody. There was good qualitative correlation between the two tests.

AB - An enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibody to border disease virus (BDV) in sheep serum. A monoclonal antibody bound to 96-well microplates was used to capture antigen from detergent-solubilised BDV-infected cells. Single dilutions of test sera were then added to wells containing bound BDV antigen and control wells containing uninfected cell lysates. Specific antibody to BDV was detected by an anti-ovine IgG antiserum conjugated with horseradish peroxidase and the results expressed as ELISA units with reference to a standard curve. Sequential sera from 16 experimentally infected sheep and single sera from 103 sheep involved in a field outbreak were tested in the ELISA and for neutralising antibody. There was good qualitative correlation between the two tests.

KW - animals

KW - Antibodies, Monoclonal

KW - Antibodies, Viral

KW - Border Disease

KW - Enzyme-Linked Immunosorbent Assay

KW - horseradish peroxidase

KW - Immunoglobulins

KW - Neutralization Tests

KW - pestvirus

KW - sheep

U2 - 10.1016/0378-1135(91)90067-P

DO - 10.1016/0378-1135(91)90067-P

M3 - Article

SN - 0378-1135

VL - 28

SP - 327

EP - 333

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 4

ER -

A monoclonal antibody capture ELISA to detect antibody to border disease virus in sheep serum

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this