A mouse model of intestinal stem cell function and regeneration

E M Slorach, F C Campbell, J R Dorin

Research output: Contribution to journalArticlepeer-review

Abstract

We present here an in vivo mouse model for intestinal stem cell function and differentiation that uses postnatal intestinal epithelial cell aggregates to generate a differentiated murine small intestinal mucosa with full crypt-villus architecture. The process of neomucosal formation is highly similar to that of intestinal regeneration. Both in vivo grafting and primary culture of these cells reveal two different epithelial cell populations, which display properties consistent with intestinal epithelial transit amplifying and stem cell populations. Using this model system with a mixture of wild-type and transgene marked cells, we have shown that neomucosae originally develop from single aggregates, but that over time the mucosae fuse to form chimaeric mucosae. Despite fusion, the chimaeric mucosae maintain crypt clonality and villus polyclonality, demonstrating that clonal segregation persists during intestinal epithelial regeneration.
Original languageEnglish
Pages (from-to)3029-38
Number of pages10
JournalJournal of Cell Science
Volume112
Issue number18
Publication statusPublished - 1999

Keywords

  • Animals
  • Animals, Newborn
  • Cell Differentiation
  • Cell Division
  • Chimera
  • Culture Techniques
  • Epithelial Cells
  • Intestinal Mucosa
  • Intestine, Small
  • Mice
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Models, Biological
  • Organoids
  • Regeneration
  • Stem Cell Transplantation
  • Stem cells

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