A mouse monoclonal antibody against Epstein-Barr virus envelope glycoprotein 350 prevents infection both in vitro and in vivo

Tanzina Haque; Ingolfur Johannessen; Dilani Dombagoda; Chandrya Sengupta; David M Burns; Pru Bird; Geoff Hale; Giorgina Mieli-Vergani; Dorothy H Crawford

doi:10.1086/505912

A mouse monoclonal antibody against Epstein-Barr virus envelope glycoprotein 350 prevents infection both in vitro and in vivo

Tanzina Haque, Ingolfur Johannessen, Dilani Dombagoda, Chandrya Sengupta, David M Burns, Pru Bird, Geoff Hale, Giorgina Mieli-Vergani, Dorothy H Crawford

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A mouse monoclonal antibody (MAb) against Epstein-Barr virus (EBV) envelope glycoprotein 350, 72A1, inhibited EBV infection of B lymphocytes in vitro. When severe combined immunodeficient mice were injected with EBV-seronegative donors' peripheral-blood mononuclear cells and challenged with EBV, 72A1 MAb prevented development of EBV-positive tumors: none of the test mice (0/12) developed EBV-positive tumors. In contrast, 67% (8/12) of control mice developed EBV-positive tumors (P=.001). Purified 72A1 MAb was infused into 1 healthy adult and 4 EBV-seronegative children after liver transplant. No adverse reactions were seen in the adult or in 3 of the transplant recipients. The remaining patient developed a hypersensitivity reaction, thus underlining the need to humanize the MAb.

Original language	English
Pages (from-to)	584-7
Number of pages	4
Journal	The Journal of Infectious Diseases
Volume	194
Issue number	5
DOIs	https://doi.org/10.1086/505912
Publication status	Published - 1 Sept 2006

Keywords / Materials (for Non-textual outputs)

Adult
Animals
Antibodies, Monoclonal
Burkitt Lymphoma
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Humans
Mice
Mice, SCID
Transplantation Immunology

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10.1086/505912

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@article{08ccb198e1d94343b0eec3e29dbb6b75,

title = "A mouse monoclonal antibody against Epstein-Barr virus envelope glycoprotein 350 prevents infection both in vitro and in vivo",

abstract = "A mouse monoclonal antibody (MAb) against Epstein-Barr virus (EBV) envelope glycoprotein 350, 72A1, inhibited EBV infection of B lymphocytes in vitro. When severe combined immunodeficient mice were injected with EBV-seronegative donors' peripheral-blood mononuclear cells and challenged with EBV, 72A1 MAb prevented development of EBV-positive tumors: none of the test mice (0/12) developed EBV-positive tumors. In contrast, 67% (8/12) of control mice developed EBV-positive tumors (P=.001). Purified 72A1 MAb was infused into 1 healthy adult and 4 EBV-seronegative children after liver transplant. No adverse reactions were seen in the adult or in 3 of the transplant recipients. The remaining patient developed a hypersensitivity reaction, thus underlining the need to humanize the MAb.",

keywords = "Adult, Animals, Antibodies, Monoclonal, Burkitt Lymphoma, Epstein-Barr Virus Infections, Herpesvirus 4, Human, Humans, Mice, Mice, SCID, Transplantation Immunology",

author = "Tanzina Haque and Ingolfur Johannessen and Dilani Dombagoda and Chandrya Sengupta and Burns, {David M} and Pru Bird and Geoff Hale and Giorgina Mieli-Vergani and Crawford, {Dorothy H}",

year = "2006",

month = sep,

day = "1",

doi = "10.1086/505912",

language = "English",

volume = "194",

pages = "584--7",

journal = "The Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

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TY - JOUR

T1 - A mouse monoclonal antibody against Epstein-Barr virus envelope glycoprotein 350 prevents infection both in vitro and in vivo

AU - Haque, Tanzina

AU - Johannessen, Ingolfur

AU - Dombagoda, Dilani

AU - Sengupta, Chandrya

AU - Burns, David M

AU - Bird, Pru

AU - Hale, Geoff

AU - Mieli-Vergani, Giorgina

AU - Crawford, Dorothy H

PY - 2006/9/1

Y1 - 2006/9/1

N2 - A mouse monoclonal antibody (MAb) against Epstein-Barr virus (EBV) envelope glycoprotein 350, 72A1, inhibited EBV infection of B lymphocytes in vitro. When severe combined immunodeficient mice were injected with EBV-seronegative donors' peripheral-blood mononuclear cells and challenged with EBV, 72A1 MAb prevented development of EBV-positive tumors: none of the test mice (0/12) developed EBV-positive tumors. In contrast, 67% (8/12) of control mice developed EBV-positive tumors (P=.001). Purified 72A1 MAb was infused into 1 healthy adult and 4 EBV-seronegative children after liver transplant. No adverse reactions were seen in the adult or in 3 of the transplant recipients. The remaining patient developed a hypersensitivity reaction, thus underlining the need to humanize the MAb.

AB - A mouse monoclonal antibody (MAb) against Epstein-Barr virus (EBV) envelope glycoprotein 350, 72A1, inhibited EBV infection of B lymphocytes in vitro. When severe combined immunodeficient mice were injected with EBV-seronegative donors' peripheral-blood mononuclear cells and challenged with EBV, 72A1 MAb prevented development of EBV-positive tumors: none of the test mice (0/12) developed EBV-positive tumors. In contrast, 67% (8/12) of control mice developed EBV-positive tumors (P=.001). Purified 72A1 MAb was infused into 1 healthy adult and 4 EBV-seronegative children after liver transplant. No adverse reactions were seen in the adult or in 3 of the transplant recipients. The remaining patient developed a hypersensitivity reaction, thus underlining the need to humanize the MAb.

KW - Adult

KW - Animals

KW - Antibodies, Monoclonal

KW - Burkitt Lymphoma

KW - Epstein-Barr Virus Infections

KW - Herpesvirus 4, Human

KW - Humans

KW - Mice

KW - Mice, SCID

KW - Transplantation Immunology

U2 - 10.1086/505912

DO - 10.1086/505912

M3 - Article

C2 - 16897655

SN - 0022-1899

VL - 194

SP - 584

EP - 587

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 5

ER -

A mouse monoclonal antibody against Epstein-Barr virus envelope glycoprotein 350 prevents infection both in vitro and in vivo

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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