A multi-modal, multi-photon microscope for biological imaging

Rabah Mouras; Andrew Downes; Grigore Rischitor; Meropi Mari; Alistair Elfick

doi:10.1117/12.844236

A multi-modal, multi-photon microscope for biological imaging

Rabah Mouras, Andrew Downes, Grigore Rischitor, Meropi Mari, Alistair Elfick

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.

Original language	English
Title of host publication	Proceedings of SPIE Volume 7569
Subtitle of host publication	Multiphoton Microscopy in the Biomedical Sciences X
Editors	A Periasamy, PTC So, K Konig
Place of Publication	BELLINGHAM
Publisher	SPIE
Pages	-
Number of pages	6
Volume	7569
ISBN (Print)	9780819479655
DOIs	https://doi.org/10.1117/12.844236
Publication status	Published - Jan 2010
Event	Conference on Multiphoton Microscopy in the Biomedical Sciences X - San Francisco Duration: 24 Jan 2010 → 26 Jan 2010

Conference

Conference	Conference on Multiphoton Microscopy in the Biomedical Sciences X
City	San Francisco
Period	24/01/10 → 26/01/10

Access to Document

10.1117/12.844236

A MULTI MODAL TWO PHOTON/CARS IMAGING SYSTEM
Frame, M., Brunton, V., Downes, A. & Elfick, A.
UK-based charities
1/04/11 → 31/03/12
Project: Research
RCUK Fellowship
Smith, S., Anderton, S., Bagnaninchi, P., Blythe, R., Calvert, J., Downes, A., Frame, M., Hay, D., Popovic, N. & Rambaut, A.
EPSRC, STFC
1/10/06 → 30/06/13
Project: Research

Cite this

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title = "A multi-modal, multi-photon microscope for biological imaging",

abstract = "We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.",

author = "Rabah Mouras and Andrew Downes and Grigore Rischitor and Meropi Mari and Alistair Elfick",

year = "2010",

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language = "English",

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editor = "A Periasamy and PTC So and K Konig",

booktitle = "Proceedings of SPIE Volume 7569",

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note = "Conference on Multiphoton Microscopy in the Biomedical Sciences X ; Conference date: 24-01-2010 Through 26-01-2010",

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Mouras, R, Downes, A, Rischitor, G, Mari, M & Elfick, A 2010, A multi-modal, multi-photon microscope for biological imaging. in A Periasamy, PTC So & K Konig (eds), Proceedings of SPIE Volume 7569 : Multiphoton Microscopy in the Biomedical Sciences X. vol. 7569, SPIE, BELLINGHAM, pp. -, Conference on Multiphoton Microscopy in the Biomedical Sciences X, San Francisco, 24/01/10. https://doi.org/10.1117/12.844236

A multi-modal, multi-photon microscope for biological imaging. / Mouras, Rabah; Downes, Andrew; Rischitor, Grigore et al.
Proceedings of SPIE Volume 7569 : Multiphoton Microscopy in the Biomedical Sciences X. ed. / A Periasamy; PTC So; K Konig. Vol. 7569 BELLINGHAM: SPIE, 2010. p. -.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - A multi-modal, multi-photon microscope for biological imaging

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AU - Mari, Meropi

AU - Elfick, Alistair

PY - 2010/1

Y1 - 2010/1

N2 - We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.

AB - We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.

U2 - 10.1117/12.844236

DO - 10.1117/12.844236

M3 - Conference contribution

SN - 9780819479655

VL - 7569

SP - -

BT - Proceedings of SPIE Volume 7569

A2 - Periasamy, A

A2 - So, PTC

A2 - Konig, K

PB - SPIE

CY - BELLINGHAM

T2 - Conference on Multiphoton Microscopy in the Biomedical Sciences X

Y2 - 24 January 2010 through 26 January 2010

ER -

A multi-modal, multi-photon microscope for biological imaging

Abstract

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Projects

A MULTI MODAL TWO PHOTON/CARS IMAGING SYSTEM

RCUK Fellowship

Cite this