A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging

Antonio Fernandez, Nicola Kielland, Ashraff Makda, Neil O. Carragher, M. Carmen González-garcía, Laura Espinar-barranco, Juan A. González-vera, Angel Orte, Rodolfo Lavilla, Marc Vendrell

Research output: Contribution to journalArticlepeer-review

Abstract

We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.
Original languageEnglish
JournalRSC Chemical Biology
Early online date25 Aug 2022
DOIs
Publication statusE-pub ahead of print - 25 Aug 2022

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