A multiplex PCR that discriminates between Trypanosoma brucei brucei and zoonotic T-b. rhodesiense

Kim Picozzi, Mark Carrington, Susan C. Welburn

Research output: Contribution to journalArticlepeer-review

Abstract

Two subspecies of Trypanosoma brucei s.l. co-exist within the animal populations of Eastern Africa; T. b. brucei a parasite which only infects livestock and wildlife and T b. rhodesiense a zoonotic parasite which infects domestic livestock, wildlife, and which in humans, results in the disease known as Human African Trypanosomiasis (HAT) or sleeping sickness. In order to assess the risk posed to humans from HAT it is necessary to identify animals harbouring potentially human infective parasites. The multiplex PCR method described here permits differentiation of human and non-human infective parasites T b. rhodesiense and T b. brucei based on the presence or absence of the SRA gene (specific for East African T b. rhodesiense), inclusion of GPI-PLC as an internal control indicates whether sufficient genomic material is present for detection of a single copy T brucei gene in the PCR reaction. (c) 2007 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalExperimental Parasitology
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • POLYMERASE-CHAIN-REACTION
  • SLEEPING-SICKNESS
  • PHOSPHOLIPASE-C
  • HUMAN SERUM
  • GENE
  • RESISTANCE
  • DNA
  • EXPRESSION
  • LOCALIZATION
  • UGANDA

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