TY - JOUR
T1 - A new format of electrodes for the electrochemical reduction of cytochromes P450
AU - Shumyantseva, Victoria V
AU - Bulko, Tatiana V
AU - Samenkova, Natalia F
AU - Kuznetsova, Galina P
AU - Usanov, Sergei A
AU - Schulze, Holger
AU - Bachmann, Till T
AU - Schmid, Rolf D
AU - Archakov, Alexander I
PY - 2006
Y1 - 2006
N2 - New approach to the electrochemical reduction of cytochromes P450 (P450s, CYPs) at electrodes chemically modified with appropriate substrates for P450s ("reverse" electrodes) was proposed. The method is based on the analysis of cyclic voltammograms, square-wave voltammograms and amperograms with subsequent determination of electrochemical characteristics such as catalytic current and redox potential. The sensitivity of proposed method is 0.2-1 nmol P450/electrode. The changes of maximal current and of redox potentials in square-wave voltammograms as well as the changes of catalytic current in amperometric experiments proved to be informative and reliable. Planar regime of screen-printed electrodes (strip-type sensors) enabled to utilise 20-60 microl of electrolyte volume. The enzyme-substrate pairs P450 2B4/benzphetamine and P450scc/cholesterol were investigated. Electrochemical parameters of electrodes with unspecific P450 substrates differed considerably from electrodes with appropriate substrates.
AB - New approach to the electrochemical reduction of cytochromes P450 (P450s, CYPs) at electrodes chemically modified with appropriate substrates for P450s ("reverse" electrodes) was proposed. The method is based on the analysis of cyclic voltammograms, square-wave voltammograms and amperograms with subsequent determination of electrochemical characteristics such as catalytic current and redox potential. The sensitivity of proposed method is 0.2-1 nmol P450/electrode. The changes of maximal current and of redox potentials in square-wave voltammograms as well as the changes of catalytic current in amperometric experiments proved to be informative and reliable. Planar regime of screen-printed electrodes (strip-type sensors) enabled to utilise 20-60 microl of electrolyte volume. The enzyme-substrate pairs P450 2B4/benzphetamine and P450scc/cholesterol were investigated. Electrochemical parameters of electrodes with unspecific P450 substrates differed considerably from electrodes with appropriate substrates.
KW - Biosensing Techniques
KW - Cytochrome P-450 Enzyme System
KW - Electrochemistry
KW - Electrodes
KW - Oxidation-Reduction
U2 - 10.1016/j.jinorgbio.2006.03.007
DO - 10.1016/j.jinorgbio.2006.03.007
M3 - Article
C2 - 16697466
SN - 0162-0134
VL - 100
SP - 1353
EP - 1357
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 8
ER -