A new PETase from the human saliva metagenome and its functional modification via genetic code expansion in bacteria

Mirren F. M. White, Stephen Wallace*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The discovery and engineering of new plastic degrading enzymes is an important challenge in chemical biotechnology to enable transition to a more sustainable and circular plastics economy. This field has so far yielded a range of enzymes and microbial pathways for the recycling and valorization of plastic waste. New research from Uttamapinant et al. reports the discovery of a novel polyethylene terephthalate (PET) hydrolase from the human saliva metagenome that displays improved properties and catalytic performance over previously characterized PET hydrolases (PETases). The authors also demonstrate the site-specific incorporation of a photocaged unnatural amino acid, 2,3-diaminopropionic acid (DAP), which upon photodecaging enables covalent binding of DAP to the PET surface. Thus, this work highlights metagenomic datasets as an untapped source of new PET degrading enzymes and the chemical modification of PETases via genetic code expansion, enabling new biotechnologies for the circular plastics economy.
Original languageEnglish
Article numbere202216963
Number of pages3
JournalAngewandte Chemie International Edition
Volume62
Issue number12
Early online date2 Jan 2023
DOIs
Publication statusPublished - 13 Mar 2023

Keywords / Materials (for Non-textual outputs)

  • biocatalysis
  • degradation
  • genetic code expansion
  • hydrolases
  • plastics

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