A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models

Alisia M. Sim; Nabil A. Rashdan; Lin Cui; Alastair J. Moss; Fabio Nudelman; Marc R. Dweck; Vicky E. Macrae; Alison N. Hulme

doi:10.1038/s41598-018-35454-9

A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models

Alisia M. Sim, Nabil A. Rashdan, Lin Cui, Alastair J. Moss, Fabio Nudelman, Marc R. Dweck, Vicky E. Macrae, Alison N. Hulme

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.

Original language	English
Pages (from-to)	17360
Journal	Scientific Reports
Volume	8
Issue number	1
Early online date	26 Nov 2018
DOIs	https://doi.org/10.1038/s41598-018-35454-9
Publication status	Published - 26 Nov 2018

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10.1038/s41598-018-35454-9

A novel fluorescein-bisphosphonate based diagnostic
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title = "A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models",

abstract = "A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans. ",

author = "Sim, {Alisia M.} and Rashdan, {Nabil A.} and Lin Cui and Moss, {Alastair J.} and Fabio Nudelman and Dweck, {Marc R.} and Macrae, {Vicky E.} and Hulme, {Alison N.}",

year = "2018",

month = nov,

day = "26",

doi = "10.1038/s41598-018-35454-9",

language = "English",

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pages = "17360",

journal = "Scientific Reports",

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T1 - A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models

AU - Sim, Alisia M.

AU - Rashdan, Nabil A.

AU - Cui, Lin

AU - Moss, Alastair J.

AU - Nudelman, Fabio

AU - Dweck, Marc R.

AU - Macrae, Vicky E.

AU - Hulme, Alison N.

PY - 2018/11/26

Y1 - 2018/11/26

N2 - A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.

AB - A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.

U2 - 10.1038/s41598-018-35454-9

DO - 10.1038/s41598-018-35454-9

M3 - Article

C2 - 30478332

SN - 2045-2322

VL - 8

SP - 17360

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models

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