A novel high-content phenotypic screen to identify inhibitors of mitochondrial DNA maintenance in trypanosomes

Research output: Contribution to journalArticlepeer-review

Abstract

Kinetoplastid parasites cause diverse neglected diseases in humans and livestock, with an urgent need for new treatments. Survival of kinetoplastids depends on their uniquely structured mitochondrial genome (kDNA), the eponymous kinetoplast. Here we report development of a high-content screen for pharmacologically induced kDNA loss, based on specific staining of parasites and automated image analysis. As proof-of-concept we screened a diverse set of ∼14,000 small molecules and exemplify a validated hit as a novel kDNA-targeting compound
Original languageEnglish
JournalAntimicrobial Agents and Chemotherapy
Early online date6 Dec 2021
DOIs
Publication statusE-pub ahead of print - 6 Dec 2021

Keywords

  • High-throughput screening
  • high-content screening
  • trypanosomatids
  • kinetoplast
  • kDNA
  • mitochondia

Fingerprint

Dive into the research topics of 'A novel high-content phenotypic screen to identify inhibitors of mitochondrial DNA maintenance in trypanosomes'. Together they form a unique fingerprint.

Cite this