A novel immunohistochemical method for estimating cell cycle phase distribution in ovarian serous neoplasms: implications for the histopathological assessment of paraffin-embedded specimens

I S Scott, T M Heath, L S Morris, S M Rushbrook, K Bird, S L Vowler, M J Arends, N Coleman

Research output: Contribution to journalArticlepeer-review

Abstract

We have investigated whether immunohistochemical markers can identify differences in cell cycle phase distribution in ovarian serous neoplasms, including borderline tumours of different grades. Sections of normal ovary (n=18), serous cystadenoma (n=21), borderline serous tumours (n=21) and serous cystadenocarcinoma (n=15) were analysed by immunohistochemistry using markers of cell cycle entry (Mcm-2) and cell cycle phase, including cyclin D1 (mid-to-late G1), cyclin A (S phase), cyclin B1 (G2 phase) and phosphohistone H3 (mitosis). Double-labelling confocal microscopy confirmed marker phase specificity and phase estimations were corroborated by flow cytometry. On progression from normal ovary through serous cystadenoma and borderline tumours to cystadenocarcinomas, expression of Mcm-2 (P
Original languageEnglish
Pages (from-to)1583-90
Number of pages8
JournalBritish Journal of Cancer
Volume90
Issue number8
DOIs
Publication statusPublished - 19 Apr 2004

Keywords

  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Transformation, Neoplastic
  • Cystadenocarcinoma, Serous
  • Disease Progression
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Microscopy, Confocal
  • Ovarian Neoplasms
  • Precancerous Conditions
  • Specimen Handling
  • Tumor Markers, Biological

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