A Novel, Low-Volume Method for Organ Culture of Embryonic Kidneys That Allows Development of Cortico-Medullary Anatomical Organization

David Sebinger, Mathieu Unbekandt, Veronika Ganeva, Andreas Offenbauer, Carsten Werner, Jamie Davies

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle.
Original languageEnglish
Article numbere10550
Number of pages10
JournalPLoS ONE
Volume5
Issue number5
DOIs
Publication statusPublished - May 2010

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