A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection

Daniel Evans*, Konstantinos I. Papadimitriou, Nikolaos Vasilakis, Panagiotis Pantelidis, Peter Kelleher, Hywel Morgan, Themistoklis Prodromakis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H2 O2 depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.

Original languageEnglish
Article number4011
JournalSensors (Switzerland)
Volume18
Issue number11
DOIs
Publication statusPublished - 17 Nov 2018

Keywords

  • Cytokine detection
  • EELISA
  • Lab-on-PCB
  • Microfluidics
  • PCB biosensors
  • Point-of-care diagnostics

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