A novel variant of Inpp5f is imprinted in brain, and its expression is correlated with differential methylation of an internal CpG island

JD Choi, LA Underkoffler, AJ Wood, JN Collins, PT Williams, JA Golden, EF Schuster, KM Loomes, RJ Oakey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Using a tissue-specific microarray screen in combination with chromosome anomalies in the mouse, we identified a novel imprinted gene, Inpp5f_v2 on mouse chromosome 7. Characterization of this gene reveals a 3.2-kb transcript that is paternally expressed in the brain. Inpp5f_v2 is a variant of the related 4.7-kb transcript, Inpp5f, an inositol phosphatase gene that is biallelically expressed in the mouse. Inpp5f_v2 uses an alternative transcriptional start site within an intron of Inpp5f and thus has a unique alternative first exon. Whereas other imprinted transcripts have a unique first exon located within intron 1 of a longer transcript variant (such as at the Gnas and WT1 loci), Inpp5f_v2 is the first example of which we are aware in which the alternative first exon of an imprinted gene is embedded in a downstream intron (intron 15) of a transcript variant. The CpG island associated with the nonimprinted Inpp5f gene is hypomethylated on both alleles, a finding consistent with biallelic expression, whereas the CpG island present 5' of Inpp5f_v2 is differentially methylated on the maternal versus paternal alleles consistent with its imprinting status.

Original languageEnglish
Pages (from-to)5514-5522
Number of pages9
JournalMolecular and Cellular Biology
Volume25
Issue number13
Publication statusPublished - Jul 2005

Keywords

  • BECKWITH-WIEDEMANN-SYNDROME
  • MOUSE GNAS LOCUS
  • MESSENGER-RNA
  • GENE
  • DOMAIN
  • H19
  • CLUSTER
  • GENOME
  • REGION
  • IDENTIFICATION

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