A Nucleoporin Docks Protein Phosphatase 1 to Direct Meiotic Chromosome Segregation and Nuclear Assembly

Neil Hattersley, Dhanya Cheerambathur, Mark Moyle, Marine Stefanutti, Amelia Richardson, Kian-Yong Lee, Julien Dumont, Karen Oegema, Arshad Desai

Research output: Contribution to journalArticlepeer-review

Abstract

During M-phase entry in metazoans with open mitosis, the concerted action of mitotic kinases disassembles nuclei and promotes assembly of kinetochores-the primary microtubule attachment sites on chromosomes. At M-phase exit, these major changes in cellular architecture must be reversed. Here, we show that the conserved kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of protein phosphatase 1 (PP1c) to direct kinetochore disassembly-dependent chromosome segregation during oocyte meiosis I and nuclear assembly during the transition from M phase to interphase. During oocyte meiosis I, MEL-28-PP1c disassembles kinetochores in a timely manner to promote elongation of the acentrosomal spindles that segregate homologous chromosomes. During nuclear assembly, MEL-28 recruits PP1c to the periphery of decondensed chromatin, where it directs formation of a functional nuclear compartment. Thus, a pool of phosphatase activity associated with a kinetochore-localized nucleoporin contributes to two key events that occur during M-phase exit in metazoans: kinetochore disassembly and nuclear reassembly.

Original languageEnglish
Pages (from-to)463-477
Number of pages15
JournalDevelopmental Cell
Volume38
Issue number5
DOIs
Publication statusPublished - 12 Sep 2016

Keywords

  • Animals
  • Caenorhabditis elegans/genetics
  • Caenorhabditis elegans Proteins/genetics
  • Chromosome Segregation/genetics
  • Humans
  • Kinetochores/metabolism
  • Meiosis/genetics
  • Nuclear Envelope/genetics
  • Nuclear Pore Complex Proteins/genetics
  • Nuclear Proteins/genetics
  • Oocytes/growth & development
  • Protein Interaction Maps/genetics
  • Receptors, Neuropeptide Y/genetics
  • ELYS
  • MEL-28
  • PP1 docking
  • centromere
  • chromosome segregation
  • kinetochore
  • nuclear pore assembly
  • protein phosphatase 1

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