A pathway for mitotic chromosome formation

Johan H. Gibcus, Kumiko Samejima, Anton Goloborodko, Itaru Samejima, Natalia Naumova, Johannes Nuebler, Masato T Kanemaki, Linfeng Xie, James R. Paulson, William C. Earnshaw*, Leonid A Mirny, Job Dekker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Mitotic chromosomes fold as compact arrays of chromatin loops.To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed.During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops.The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold.The size of helical turns progressively increases to ~12megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding.

Original languageEnglish
Article numbereaao6135
Number of pages14
Issue number6376
Early online date18 Jan 2018
Publication statusPublished - 9 Feb 2018


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