TY - GEN
T1 - A PCB-based electronic ELISA system for rapid, portable infectious disease diagnosis
AU - Papadimitriou, Konstantinos I.
AU - Evans, Daniel
AU - Morgan, Hywel
AU - Prodromakis, Themistoklis
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2017/1/26
Y1 - 2017/1/26
N2 - In this paper an amperometric electrochemical detection method is demonstrated and implemented using exclusively Printed Circuit Board (PCB)-based technologies. A portable, reconfigurable, multichannel amperometric data-acquisition board has been designed and fabricated, dedicated to the measurement of current-input signals delivered by the PCB-based biosensor. The electronic read-out circuit is able to provide constant biasing voltages to the amperometric sensor, measure in real-time the sensor's output currents, digitise them using high-accuracy Analog-to-Digital Converters (ADCs) and send the binary data to the user either through a USB2.0 interface or via an on-board TFT touch-screen. In order to validate the robustness and accuracy of the combined system, proof-of-concept amperometric experiments have taken place using our custom-made PCB-based system and standard electrochemical substrates. The results obtained have been cross-validated by means of standard colorimetric analysis and their differences have been highlighted and analyzed.
AB - In this paper an amperometric electrochemical detection method is demonstrated and implemented using exclusively Printed Circuit Board (PCB)-based technologies. A portable, reconfigurable, multichannel amperometric data-acquisition board has been designed and fabricated, dedicated to the measurement of current-input signals delivered by the PCB-based biosensor. The electronic read-out circuit is able to provide constant biasing voltages to the amperometric sensor, measure in real-time the sensor's output currents, digitise them using high-accuracy Analog-to-Digital Converters (ADCs) and send the binary data to the user either through a USB2.0 interface or via an on-board TFT touch-screen. In order to validate the robustness and accuracy of the combined system, proof-of-concept amperometric experiments have taken place using our custom-made PCB-based system and standard electrochemical substrates. The results obtained have been cross-validated by means of standard colorimetric analysis and their differences have been highlighted and analyzed.
KW - Amperometry
KW - Analog-to-Digital Conversion
KW - Data Acquisition
KW - Electrochemical Assay
KW - ELISA
KW - PCB-Based Sensor
KW - Point-Of-Care Diagnostics
KW - TMB
UR - http://www.scopus.com/inward/record.url?scp=85014300451&partnerID=8YFLogxK
U2 - 10.1109/BioCAS.2016.7833779
DO - 10.1109/BioCAS.2016.7833779
M3 - Conference contribution
AN - SCOPUS:85014300451
T3 - Proceedings - 2016 IEEE Biomedical Circuits and Systems Conference, BioCAS 2016
SP - 252
EP - 255
BT - Proceedings - 2016 IEEE Biomedical Circuits and Systems Conference, BioCAS 2016
PB - Institute of Electrical and Electronics Engineers
T2 - 12th IEEE Biomedical Circuits and Systems Conference, BioCAS 2016
Y2 - 17 October 2016 through 19 October 2016
ER -