TY - JOUR
T1 - A perivascular origin for mesenchymal stem cells in multiple human organs
AU - Crisan, Mihaela
AU - Yap, Solomon
AU - Casteilla, Louis
AU - Chen, Chien-Wen
AU - Corselli, Mirko
AU - Park, Tea Soon
AU - Andriolo, Gabriella
AU - Sun, Bin
AU - Zheng, Bo
AU - Zhang, Li
AU - Norotte, Cyrille
AU - Teng, Pang-Ning
AU - Traas, Jeremy
AU - Schugar, Rebecca
AU - Deasy, Bridget M.
AU - Badylak, Stephen
AU - Buehring, Hans-Joerg
AU - Giacobino, Jean-Paul
AU - Lazzari, Lorenza
AU - Huard, Johnny
AU - Peault, Bruno
PY - 2008/9/11
Y1 - 2008/9/11
N2 - Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-R beta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
AB - Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-R beta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
KW - STEMCELL
UR - http://www.scopus.com/inward/record.url?scp=50849139576&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2008.07.003
DO - 10.1016/j.stem.2008.07.003
M3 - Article
SN - 1934-5909
VL - 3
SP - 301
EP - 313
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 3
ER -