A posttranscriptional pathway regulates cell wall mRNA expression in budding yeast

Stefan Bresson, Vadim Shchepachev, David Tollervey

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The fungal cell wall provides protection and structure and is an important target for antifungal compounds. A mitogen-activated protein (MAP) kinase cascade termed the cell wall integrity (CWI) pathway regulates transcriptional responses to cell wall damage. Here, we describe a posttranscriptional pathway that plays an important complementary role. We report that the RNA-binding proteins (RBPs) Mrn1 and Nab6 specifically target the 3′ UTRs of a largely overlapping set of cell wall-related mRNAs. These mRNAs are downregulated in the absence of Nab6, indicating a function in target mRNA stabilization. Nab6 acts in parallel to CWI signaling to maintain appropriate expression of cell wall genes during stress. Cells lacking both pathways are hypersensitive to antifungal compounds targeting the cell wall. Deletion of MRN1 partially alleviates growth defects associated with Δnab6, and Mrn1 has an opposing function in mRNA destabilization. Our results uncover a posttranscriptional pathway that mediates cellular resistance to antifungal compounds.
Original languageEnglish
Article number112184
Number of pages28
JournalCell Reports
Issue number3
Early online date28 Feb 2023
Publication statusPublished - 28 Mar 2023

Keywords / Materials (for Non-textual outputs)

  • posttranscriptional gene regulation
  • saccharomyces cerevisiae
  • UV-crosslinking
  • stress response
  • RNA-binding proteins
  • genetic
  • cell wall
  • antifungal
  • mRNA stability


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